Chapter 26, Problem 26.20P

(a)

Interpretation Introduction

Interpretation:

The volume of gel-filtration of given column should be determined.

Concept introduction:

The volume of column is given by the mathematical expression it is,

${\text{V}}_{\text{t}}\text{=}{\text{πr}}^{\text{2}}\text{l}$

Where,

${\text{V}}_{\text{f}}$ is volume of column

$\pi$ is 3.14

$\text{r}$ is radius of the column

$\text{l}$ is length of the column

(b)

Interpretation Introduction

Interpretation:

The ${\text{K}}_{\text{av}}$ of given gel-filtration column should be determined.

The Elution Equation;

${\text{K}}_{\text{av}}\text{=}\frac{{\text{V}}_{\text{r}}{\text{-V}}_{\text{0}}}{{\text{V}}_{\text{m}}{\text{-V}}_{\text{0}}}$

Where,

${\text{V}}_{\text{m}}$ is total volume of mobile phase.

${\text{V}}_{\text{r}}$ is volume of known solute.

${\text{V}}_{\text{0}}$ is initial volume.