Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 26, Problem 1S
E. coli lysogens derived from infection by phage λ can be induced to form progeny viruses by exposure to radiation. The inductive event is the destruction of a repressor protein that keeps the prophage genome unexpressed. What might be the normal role of the protein that recognizes and destroys the λ repressor?
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The linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions.
1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?
Chapter 26 Solutions
Biology
Ch. 26.1 - Prob. 1LOCh. 26.1 - Prob. 2LOCh. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.3 - Prob. 1LOCh. 26.3 - Describe how viruses can contribute DNA to their...Ch. 26.4 - Describe the differences between acute and...Ch. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.5 - Prob. 1LO
Ch. 26 - Prob. 1UCh. 26 - Prob. 2UCh. 26 - Prob. 3UCh. 26 - Prob. 4UCh. 26 - Why is a drug that blocks HIV binding to one of...Ch. 26 - Prob. 6UCh. 26 - Prob. 7UCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - Prob. 3ACh. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - Prob. 6ACh. 26 - Prob. 7ACh. 26 - E. coli lysogens derived from infection by phage ...Ch. 26 - Prob. 2SCh. 26 - Prob. 3SCh. 26 - Prob. 4SCh. 26 - Prob. 5S
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- The size of a DNA fragment that can be inserted into an unmodified λ vector is very limited. Large segment in the central region of the λ DNA molecule can be removed without affecting the ability of the phage to infect Escherichia coli cells. However, the deletion of this non- essential region does affect the life cycle of the bacteriophage. Explain this statement.arrow_forwardWhen various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid plaques. (b) For mutant λ phages that can only form clear plaques, give two different types of mutation in the phage that can explain the clear plaque phenotype.arrow_forwardOne of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forward
- The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in: a) The establishment of lysogeny b) The establishment of a lytic life cyclearrow_forwardIn a study, bacterial culture of E. coli was infected with bacteriophage. How- ever, these cultures were protected from phage infection. Interestingly, it was found that the resistance is due to endonudeases present in E. coli, which deaved the phage DNA. How is the E. coli genomic DNA protected from the action of these endonuclease enzymes?arrow_forwardwhen various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forward
- What is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardThe Pfizer Covid-19 vaccine is one of the vaccines currently being rolled out for mass vaccination in South Africa to protect the population against SARS CoV-2, and the active compound in this vaccine is an mRNA molecule. a) The starting material for productio of this vaccine is a copy of the coronavirus spike protein gene cloned into a ppasmid. List the main experimental steps in the vaccine production process, to go from a plamid to an mRNA molecule. b) Which molecular processes in the cell will allow an injected strand of mRNA to produce anti-Covid immunity in the vaccine recipient?arrow_forward
- In immediate-early transcription of the phage genome, the N protein works as an antiterminator to allow transcription of the delayed early genes. After this, if the bacterial cell that this lambda phage infects is robustly healthy, it will make many proteases that overwhelm the efforts of the clll protein in protecting the cll protein from degradation. If the cll protein is degraded, protein will block transcription from the PRM promoter and promote the lysis the cro pathway. If the lysogenic pathway prevails, the cll protein is protected P-RM promoter. This results in the which blocks transcription from P-RM cro . Upon integration, the prop until environmental conditions induce the lytic pathway. [Select] cl cill cro N P-L P-RM P-RE transcription from the protein being made, nscription from s host chromosomearrow_forwardE. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interferencearrow_forwardMost bacterial repressor proteins are allosteric. Which of the following binds with the repressor to alter its conformation? A) promoter B) corepressor C) ATParrow_forward
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