Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 25.1, Problem 1CYP
Summary Introduction
To determine:
The structure and function of hemagglutinin and neuraminidase spikes in influenza viruses.
Introduction:
Hemagglutinin and neuraminidase are glycoprotein antigens present in influenza viruses, that contribute to the virulence of the virus.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 25 Solutions
Foundations in Microbiology
Ch. 25.1 - Prob. 1ELOCh. 25.1 - Understand what is meant by antigenic shift and...Ch. 25.1 - Prob. 3ELOCh. 25.1 - Prob. 4ELOCh. 25.1 - Prob. 5ELOCh. 25.1 - Prob. 1CYPCh. 25.1 - What is unusual about the genome of influenza...Ch. 25.1 - Explain how antigenic shift and drift differ and...Ch. 25.1 - Explain the course of infection and disease and...Ch. 25.1 - Explain generally how flu vaccines are prepared....
Ch. 25.1 - Prob. 6CYPCh. 25.1 - Prob. 7CYPCh. 25.2 - Recall the enveloped viruses possessing a...Ch. 25.2 - Prob. 7ELOCh. 25.2 - Prob. 8ELOCh. 25.2 - Prob. 9ELOCh. 25.2 - Describe the structural characteristics common to...Ch. 25.2 - Describe the steps in the production of...Ch. 25.2 - Prob. 10CYPCh. 25.2 - Name two examples of Paramyxovirus and describe...Ch. 25.2 - Prob. 12CYPCh. 25.2 - Summarize the epidemiology, diagnosis, treatment,...Ch. 25.2 - Describe the epidemiological cycle in rabies.Ch. 25.2 - Which animals in the United States are most...Ch. 25.2 - Prob. 16CYPCh. 25.2 - Prob. 17CYPCh. 25.3 - Prob. 10ELOCh. 25.3 - Understand the epidemiology, diagnosis, and...Ch. 25.3 - Prob. 12ELOCh. 25.3 - Summarize the transmission and pathology of the...Ch. 25.3 - Prob. 18CYPCh. 25.3 - Prob. 19CYPCh. 25.3 - Prob. 20CYPCh. 25.3 - Prob. 21CYPCh. 25.3 - Prob. 22CYPCh. 25.3 - Outline a typical course of hepatitis C infection,...Ch. 25.4 - Prob. 14ELOCh. 25.4 - Describe the pathology of arboviral disease.Ch. 25.4 - Prob. 16ELOCh. 25.4 - Name several activities that increase the risk of...Ch. 25.4 - Prob. 25CYPCh. 25.4 - Prob. 26CYPCh. 25.4 - How is the cycle of the viruses maintained in the...Ch. 25.4 - Describe the symptoms of the encephalitis type of...Ch. 25.4 - Prob. 29CYPCh. 25.5 - Prob. 17ELOCh. 25.5 - Prob. 18ELOCh. 25.5 - Describe the structural features of the human...Ch. 25.5 - Prob. 20ELOCh. 25.5 - Prob. 21ELOCh. 25.5 - Prob. 22ELOCh. 25.5 - Prob. 23ELOCh. 25.5 - Prob. 24ELOCh. 25.5 - Prob. 25ELOCh. 25.5 - Understand the purpose of using combination...Ch. 25.5 - Describe the diseases associated with HTLV-I.Ch. 25.5 - What are retroviruses, and how are they different...Ch. 25.5 - Prob. 31CYPCh. 25.5 - Prob. 32CYPCh. 25.5 - Prob. 33CYPCh. 25.5 - Prob. 34CYPCh. 25.5 - Prob. 35CYPCh. 25.5 - Prob. 36CYPCh. 25.5 - Prob. 37CYPCh. 25.5 - Prob. 38CYPCh. 25.5 - List the major opportunistic bacterial, fungal,...Ch. 25.5 - Prob. 40CYPCh. 25.5 - Prob. 41CYPCh. 25.5 - Prob. 42CYPCh. 25.5 - What is the rationale behind the use of HAART...Ch. 25.5 - Prob. 44CYPCh. 25.5 - Prob. 45CYPCh. 25.6 - Prob. 28ELOCh. 25.6 - Describe the range of pathologies seen in...Ch. 25.6 - Prob. 30ELOCh. 25.6 - Understand the epidemiology, diagnosis, and...Ch. 25.6 - Understand why rhinovirus infections are typically...Ch. 25.6 - Describe the epidemiology and pathology of...Ch. 25.6 - Prob. 34ELOCh. 25.6 - Prob. 46CYPCh. 25.6 - Prob. 47CYPCh. 25.6 - Prob. 48CYPCh. 25.6 - Prob. 49CYPCh. 25.6 - What characteristics of enteric viruses cause them...Ch. 25.6 - Prob. 51CYPCh. 25.6 - Prob. 52CYPCh. 25.6 - List several activities that reduce the incidence...Ch. 25.6 - Prob. 54CYPCh. 25.6 - Prob. 55CYPCh. 25.6 - Prob. 56CYPCh. 25.6 - Prob. 57CYPCh. 25.6 - What viruses possess a double-stranded RNA genome...Ch. 25.7 - Prob. 35ELOCh. 25.7 - Prob. 36ELOCh. 25.7 - Prob. 37ELOCh. 25.7 - Describe the characteristics of the agents...Ch. 25.7 - Explain how bovine spongiform encephalopathy can...Ch. 25.7 - Make a flowchart to explain the mechanism of how...Ch. 25.7 - Prob. 62CYPCh. 25.7 - Prob. 63CYPCh. 25.L1 - Which receptors of the influenza virus are...Ch. 25.L1 - Prob. 2MCQCh. 25.L1 - Prob. 3MCQCh. 25.L1 - Prob. 4MCQCh. 25.L1 - Prob. 5MCQCh. 25.L1 - A common, highly diagnostic sign of measles is a....Ch. 25.L1 - Prob. 7MCQCh. 25.L1 - For which disease are active and passive...Ch. 25.L1 - Prob. 9MCQCh. 25.L1 - Prob. 10MCQCh. 25.L1 - Prob. 11MCQCh. 25.L1 - Rhinoviruses are the most common cause of a....Ch. 25.L1 - Prob. 13MCQCh. 25.L1 - Prob. 14MCQCh. 25.L1 - Prob. 15MCQCh. 25.L1 - Prob. 16MCQCh. 25.L1 - During which periods of a measles infection is a...Ch. 25.L1 - Match each of the statement below to measles or...Ch. 25.L1 - Prob. 3CSRCh. 25.L1 - Prob. 1WCCh. 25.L1 - Prob. 2WCCh. 25.L1 - Prob. 3WCCh. 25.L1 - Prob. 4WCCh. 25.L1 - Prob. 5WCCh. 25.L1 - Prob. 6WCCh. 25.L2 - a. Explain the relationship between herd immunity...Ch. 25.L2 - Prob. 2CTCh. 25.L2 - Prob. 3CTCh. 25.L2 - Prob. 4CTCh. 25.L2 - Prob. 5CTCh. 25.L2 - Prob. 6CTCh. 25.L2 - Prob. 7CTCh. 25.L2 - Prob. 8CTCh. 25.L2 - Prob. 9CTCh. 25.L2 - Prob. 10CTCh. 25.L2 - Biopsies from the liver and intestine of an...Ch. 25.L2 - Refer to figures 25.2 and 25.23, and compare and...
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