FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 25, Problem 31E
Summary Introduction
To determine: Whether Pol V is more or less processive than Pol III.
Introduction: DNA polymerase is a key enzyme that participates in the
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Extracts from the bacterium Bacillus brevis contain a peptide with antibiotic properties. This peptide forms complexes with
metal ions and seems to disrupt ion transport across the cell membranes of other bacterial species, leading to bacterial death.
The structure of the peptide has been determined from a series of observations.
(a) Complete acid hydrolysis of the peptide, followed by amino acid analysis, yielded equimolar amounts of Leu, Orn, Phe, Pro,
and Val. Orn is ornithine, an amino acid not present in proteins but present in some peptides. Orn has the structure
H
'H,N-CH,
-
CH2 CH2 CH2 -
C - COO-
NH,
(b) The molecular weight of the peptide is approximately 1,200 Da.
(c) The peptide failed to undergo hydrolysis when treated with the enzyme carboxypeptidase. This enzyme catalyzes the
hydrolysis of the carboxyl-terminal residue of a polypeptide unless the residue is Pro or the peptide does not contain a free
carboxyl group.
(d) Treatment of the intact peptide with…
At a pH equal to the isoelectric point (pl) of alanine, the net charge of alanine is zero. Two structures can be drawn that have a
net charge of zero, but the predominant form of alanine at its pl is zwitterionic.
CH3
H,N
CH3
**
H¸N-C
H
Zwitterionic
H
Uncharged
OH
Select statements that explain why alanine is predominantly zwitterionic at its pl.
pk of alanine's amino group is more than its pl.
pk of alanine's carboxyl group is more than its pl.
PK of alanine's carboxyl group is less than its pl.
pk of alanine's amino group is less than its pl.
Correct Answer
What fraction of alanine is in the completely uncharged form at its pl?
1 in 2.2 × 107
1 in 1.6 × 10²
1 in 4680
1 in 9460
How does a voltage-gated sodium channel work? Specifically, how and why does a change in voltage trigger their opening? Please be detailed
Chapter 25 Solutions
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
Ch. 25 - Prob. 1ECh. 25 - Prob. 2ECh. 25 - 3. You have discovered a drug that inhibits the...Ch. 25 - Prob. 4ECh. 25 - Prob. 5ECh. 25 - Prob. 6ECh. 25 - Prob. 7ECh. 25 - Prob. 8ECh. 25 - Prob. 9ECh. 25 - Prob. 10E
Ch. 25 - Prob. 11ECh. 25 - Prob. 12ECh. 25 - Prob. 13ECh. 25 - Prob. 14ECh. 25 - Prob. 15ECh. 25 - Prob. 16ECh. 25 - Prob. 17ECh. 25 - Prob. 18ECh. 25 - Prob. 19ECh. 25 - Prob. 20ECh. 25 - Prob. 21ECh. 25 - Prob. 22ECh. 25 - 23. Certain sites in the E. coli chromosome are...Ch. 25 - Prob. 24ECh. 25 - Prob. 25ECh. 25 - Prob. 26ECh. 25 - Prob. 27ECh. 25 - Prob. 28ECh. 25 - Prob. 29ECh. 25 - Prob. 30ECh. 25 - Prob. 31ECh. 25 - Prob. 32ECh. 25 - Prob. 33ECh. 25 - Prob. 34ECh. 25 - Prob. 35CQCh. 25 - Prob. 36CQCh. 25 - Prob. 37CQCh. 25 - Prob. 38CQCh. 25 - Prob. 39CQCh. 25 - Prob. 40CQCh. 25 - Prob. 41CQCh. 25 - Prob. 42CQCh. 25 - 43. Deinococcus radiodurans, which the Guinness...Ch. 25 - Prob. 44CQCh. 25 - Prob. 1MTE
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- When sodium ions enter a neuron during depolarization, they trigger the opening of additional voltage-gated sodium channels nearby, creating a positive feedback loop where the influx of sodium ions further depolarizes the membrane, causing even more sodium channels to open and allowing more sodium ions to enter the cell, thus sustaining the depolarization process until the action potential peaks. But how and why exactly does the influx of sodium ions trigger more sodium channels to let in more sodium? Please explainarrow_forwardDraw the chemical structure of the tripeptide, HEL (L - amino acids), at pH = 7.0. Calculate isoelectric pointarrow_forwardCan someone draw what this would look like?arrow_forward
- Calculate the standard change in Gibbs free energy, AGrxn, for the given reaction at 25.0 °C. Consult the table of thermodynamic properties for standard Gibbs free energy of formation values. NH,Cl(s) →NH; (aq) + C1 (aq) AGrxn -7.67 Correct Answer Determine the concentration of NH+ (aq) if the change in Gibbs free energy, AGrxn, for the reaction is -9.27 kJ/mol. 6.49 [NH+] Incorrect Answer kJ/mol Marrow_forwardWhat are some topics of interest that neurotoxicologists study? For example, toxin-induced seizures, brain death, and such along those lines?arrow_forwardCould you help me with the explanation of the answer to exercise 15, chapter 1 of Lehinger Question Nombramiento de estereoisómeros con dos carbonos quirales utilizando el sistema RS(R,R)El isómero del metilfenidato (Ritalin) se utiliza para tratar el trastorno por déficit de atención con hiperactividad (TDAH).(S,S)El isómero es un antidepresivo. Identifique los dos carbonos quirales en la siguiente estructura. ¿Es este el(R,R)o el(S,S)¿isómero? Dibuja el otro isómero. Nombramiento de estereoisómeros con dos carbonos quirales utilizando el sistema RS(R,R)El isómero del metilfenidato (Ritalin) se utiliza para tratar el trastorno por déficit de atención con hiperactividad (TDAH).(S,S)El isómero es un antidepresivo.arrow_forward
- The reaction A+B → C + D AG°' = -7.3 kcal/mol can be coupled with which of the following unfavorable reactions to drive it forward? A. EFG+HAG° = 5.6 kcal/mol. B. J+KZ+A AG° = 2.3 kcal/mol. C. P+RY+DAG° = 8.2 kcal/mol. D. C + T → V + W AG°' = -5.9 kcal/mol. E. AN→ Q+KAG°' = 4.3 kcal/mol.arrow_forwardWhat would be the toxicological endpoints for neurotoxicity?arrow_forwardWhat are "endpoints" in toxicology exactly? Please give an intuitive easy explanationarrow_forward
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