Quantitative Chemical Analysis 9e And Sapling Advanced Single Course For Analytical Chemistry (access Card)
Quantitative Chemical Analysis 9e And Sapling Advanced Single Course For Analytical Chemistry (access Card)
9th Edition
ISBN: 9781319090241
Author: Daniel C. Harris, Sapling Learning
Publisher: W. H. Freeman
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Chapter 25, Problem 25.34P
Interpretation Introduction

Interpretation:

General steps in developing an isocratic separation for reverse-phase chromatography with one organic solvent and temperature as variables has to be given.

Concept Introduction:

One of the most common mode of HPLC is the reversed-phase chromatography.  In reversed-phase chromatography, the mobile phase is more polar and the stationery phase is nonpolar or weakly polar.  The solute is more rapidly eluted from the column in reversed-phase chromatography as the mobile phase is polar.  Less polar mobile phase (solvent) has a high mobile phase strength.

This method is adequate to separate mixtures of low molecular mass.

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