Chapter 25, Problem 25.21P

a)

Interpretation Introduction

Interpretation:

The volume ${\text{V}}_{\text{m}}$ and time ${\text{t}}_{\text{m}}$ has to be calculated.

b)

Interpretation Introduction

Interpretation:

The order of elution has to be explained.

c)

Interpretation Introduction

Interpretation:

The retention factor for each solute has to be calculated.

Concept Introduction:

Retention factor:

The time that is necessary to elute that peak minus the minimum possible time for the travel of unretained solute through the column is called as retention factor. The retention factor is calculated using the equation,

$\text{k=}\frac{{\text{t}}_{\text{r}}{\text{-t}}_{\text{m}}}{{\text{t}}_{\text{m}}}$

Where,

${\text{t}}_{\text{r}}$ = retention time

${\text{t}}_{\text{m}}$ = minimum possible time for the travel of unretained solute through the column.

d)

Interpretation Introduction

Interpretation:

The order of elution has to be explained and the reason why the gradient goes from high to low Acetonitrile volume fraction has to be given.

To explain the order of elution

To give the reason why the gradient goes from high to low Acetonitrile volume fraction

e)

Interpretation Introduction

Interpretation:

The value of ${\text{k}}^{\text{*}}$ on column B has to be estimated.

To estimate the value of ${\text{k}}^{\text{*}}$