Quantitative Chemical Analysis 9e And Sapling Advanced Single Course For Analytical Chemistry (access Card)
Quantitative Chemical Analysis 9e And Sapling Advanced Single Course For Analytical Chemistry (access Card)
9th Edition
ISBN: 9781319090241
Author: Daniel C. Harris, Sapling Learning
Publisher: W. H. Freeman
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Chapter 25, Problem 25.37P
Interpretation Introduction

Interpretation:

The starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction has to be given.

Concept Introduction:

Reversed-phase chromatography:

One of the most common mode of HPLC is the reversed-phase chromatography.  In reversed-phase chromatography, the mobile phase is more polar and the stationery phase is nonpolar or weakly polar.  The solute is more rapidly eluted from the column in reversed-phase chromatography as the mobile phase is polar.  Less polar mobile phase (solvent) has a high mobile phase strength.

This method is adequate to separate mixtures of low molecular mass.

To give the starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction

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