ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 24.4, Problem 12WDYL
What are the three major types of capillaries associated with the nephron? Describe the location and general function of each.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 24 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 24.1 - Which structure of the urinary system forms urine,...Ch. 24.1 - What are the two means by which the kidney helps...Ch. 24.2 - What tissue composes the fibrous capsule that...Ch. 24.2 - What are the regions of the kidney that drain...Ch. 24.2 - What three anatomic structures of the kidney are...Ch. 24.3 - Prob. 6WDYLCh. 24.3 - Prob. 7WDYLCh. 24.3 - Prob. 8WDYLCh. 24.3 - Differentiate between the function of principal...Ch. 24.3 - What are the two primary cellular components of...
Ch. 24.4 - Prob. 11WDYLCh. 24.4 - What are the three major types of capillaries...Ch. 24.4 - What is the pathway of fluid filtered by the...Ch. 24.5 - How does tubular reabsorption differ from tubular...Ch. 24.5 - How are the components of the filtration membrane...Ch. 24.5 - What is normally filtered across the glomerular...Ch. 24.5 - Prob. 17WDYLCh. 24.5 - What is the value of the NFP if the glomerular...Ch. 24.5 - Prob. 19WDYLCh. 24.5 - If HPg increases, what is the effect on NFP? Is...Ch. 24.5 - Does urine production increase, decrease, or stay...Ch. 24.5 - What are the three factors that regulate...Ch. 24.5 - Prob. 23WDYLCh. 24.6 - What are the significant anatomic and physiologic...Ch. 24.6 - What is the transport maximum of a substance? How...Ch. 24.6 - Prob. 26WDYLCh. 24.6 - Why are proteins said to be transported rather...Ch. 24.6 - How does Na+ reabsorption occur? Which two...Ch. 24.6 - What is the effect of parathyroid hormone on the...Ch. 24.6 - How is the movement of H+ and HCO3 regulated by...Ch. 24.6 - Prob. 31WDYLCh. 24.6 - Prob. 32WDYLCh. 24.6 - Prob. 33WDYLCh. 24.7 - Prob. 34WDYLCh. 24.7 - Prob. 35WDYLCh. 24.8 - Prob. 36WDYLCh. 24.8 - Prob. 37WDYLCh. 24.8 - Prob. 38WDYLCh. 24.8 - Prob. 39WDYLCh. 24 - Prob. 1DYKBCh. 24 - _____ 2. When the kidneys are described as being...Ch. 24 - _____ 3. Which of the following is located within...Ch. 24 - _____ 4. All of the following are capillaries...Ch. 24 - _____ 5. Which of the following is a component of...Ch. 24 - _____ 6. If blood pressure in the glomerulus...Ch. 24 - _____ 7. Which hormone increases Na+ and water...Ch. 24 - _____ 8. If the tubular maximum is exceeded, then...Ch. 24 - _____ 9. The function unique to the nephron loop...Ch. 24 - _____ 10. If antidiuretic hormone (ADH)...Ch. 24 - Trace blood flow into and out of the kidney....Ch. 24 - Describe where filtrate, tubular fluid, and urine...Ch. 24 - Describe the anatomic components of the...Ch. 24 - Prob. 14DYKBCh. 24 - Explain how glomerular filtration rate (GFR) is...Ch. 24 - Discuss the affect of aldosterone and antidiuretic...Ch. 24 - Explain how antidiuretic hormone (ADH) is...Ch. 24 - Describe the significant differences between blood...Ch. 24 - Identify all of the following that are functions...Ch. 24 - Explain the process of micturition.Ch. 24 - Use the following paragraph to answer questions...Ch. 24 - Prob. 2CALCh. 24 - Use the following paragraph to answer questions...Ch. 24 - Martin, a young man of 20, was in a car accident...Ch. 24 - A 19-year-old male named Paul was in a diving...Ch. 24 - A patient with cancer is treated with...Ch. 24 - Prob. 2CSLCh. 24 - Males who suffer from either benign prostatic...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
- In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forward
- Why are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forward
- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning
Human Physiology: From Cells to Systems (MindTap ...BiologyISBN:9781285866932Author:Lauralee SherwoodPublisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Human Physiology: From Cells to Systems (MindTap ...
Biology
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Cengage Learning
Excretory System; Author: Amoeba Sisters;https://www.youtube.com/watch?v=q5qaGHfdmYM;License: Standard youtube license