The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500. Introduction : Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

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Answer 1

Question

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

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Students have asked these similar questions

The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Expert Solution & Answer

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Answer 3

Question

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Expert Solution & Answer

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Answer 4

Question

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Expert Solution & Answer

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See solution

Answer 5

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 6

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

Answer 7

Chapter 24, Problem 5P

5.

Summary Introduction

To determine: The amount of base pairs present in a closed-circular DNA molecule in its relaxed form that has an Lk of 500.

Introduction:

Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics. During the relaxed form, the linkage number (Lk) is equal to the number of turns found in the DNA double helix. A closed-circular DNA molecule contains an Lk of 500 in its relaxed form.

Answer 8

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 9

(a)

Summary Introduction

To determine: The way through which Lk altered, when a protein complex binds to form a nucleosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 10

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 11

(b)

Summary Introduction

To determine: The way through which Lk altered, when one strand is broken.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 12

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 13

(c)

Summary Introduction

To determine: The way through which Lk altered, when DNA gyrase and ATP are added to the DNA solution.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 14

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 15

(d).

Summary Introduction

To determine: The way through which Lk altered, when the double helix is determined by heat.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

Answer 16

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Answer 17

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Answer 18

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Answer 19

Ch. 24 - Prob. 1P Ch. 24 - Prob. 2P Ch. 24 - Prob. 3P Ch. 24 - Prob. 4P Ch. 24 - Prob. 5P Ch. 24 - Prob. 6P Ch. 24 - Prob. 7P Ch. 24 - Prob. 8P Ch. 24 - Prob. 9P Ch. 24 - Prob. 10P

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Chapter 24 Solutions

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To determine: The way through which Lk altered, when one strand is broken. Introduction: In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk.

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Chapter 24 Solutions