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Concept explainers
To compare: The melting temperature of 1-kb segment of DNA containing 20%. A residue to that of 1-kb segment of DNA containing 30%. A residue under same conditions.
Concept introduction: Melting temperature (Tm) is the temperature at which half of the DNA melts or denatures due to the breakage in the hydrogen bond that holds two strands together. Melting temperature of a DNA duplex makes changes in the structure and stability of the DNA. Melting temperature of a DNA double helix depends on the number and percentage of base-pairs present in the structure. More the G-C base-pairs present in the structure, stronger the bond and the higher would be the melting temperature to break the bond.
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Chapter 24 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
- Show a mechanism that leads to the opening of the ring below under acid-catalyzed conditions. Give the correct Fischer projection for this sugar.arrow_forwardWhat is the stereochemical relationship between B & C?arrow_forwardDon't use ai or any chat gpt will dislike okk just use accurate information okkk okkk just solve full accurate. don't use guidelines okk just did it accurate 100% sure experts solve it correct complete solutions okkk follow all instructions requirements okkkarrow_forward
- Problem 15 of 15 Submit Using the following reaction data points, construct Lineweaver-Burk plots for an enzyme with and without an inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. Using the information from this plot, determine the type of inhibitor present. 1 mM-1 1 s mM -1 [S]' V' with 10 μg per 20 54 10 36 20 5 27 2.5 23 1.25 20 Answer: |||arrow_forward12:33 CO Problem 4 of 15 4G 54% Done On the following Lineweaver-Burk -1 plot, identify the by dragging the Km point to the appropriate value. 1/V 40 35- 30- 25 20 15 10- T Км -15 10 -5 0 5 ||| 10 15 №20 25 25 30 1/[S] Г powered by desmosarrow_forward1:30 5G 47% Problem 10 of 15 Submit Using the following reaction data points, construct a Lineweaver-Burk plot for an enzyme with and without a competitive inhibitor by dragging the points to their relevant coordinates on the graph and drawing a line of best fit. 1 -1 1 mM [S]' s mM¹ with 10 mg pe 20 V' 54 10 36 > ст 5 27 2.5 23 1.25 20 Answer: |||arrow_forward
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