Chapter 23.SE, Problem 37MP

Interpretation Introduction

Interpretation:

The mechanism of each step involved in the biosynthesis of leucine from α-ketoisovaleate is to be given.

Concept introduction:

The steps involved in the biosynthesis given are i)The nucleophilic addition of –CH₂CO CoA to the carbonyl carbon ii) Hydrolysis of the intermediate iii) Elimination of water iv) Conjugate addition of water v) Oxidation by NAD⁺ vi) Decarboxylation of the β-keto ester vii) Nucleophilic attack of ammonia, loss of water and reduction

To give:

The mechanism of each step involved in the biosynthesis of leucine from α-ketoisovaleate.