Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 23.2, Problem 4COMQ
Summary Introduction
Introduction:
Protein microarrays are designed in the same way as DNA microarrays. The only difference between protein microarrays and DNA microarrays is that the silica or glass plated slide is dotted with proteins instead of DNA sequence. Protein microarrays are of two types, namely, antibody microarrays and functional protein microarrays.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 23 Solutions
Genetics: Analysis and Principles
Ch. 23.1 - 1. A DNA microarray is a slide that is dotted...Ch. 23.1 - Prob. 2COMQCh. 23.1 - 2. The purpose of a ChIP-chip assay is to...Ch. 23.1 - A gene knockout is a gene a. whose function has...Ch. 23.2 - Prob. 1COMQCh. 23.2 - Prob. 2COMQCh. 23.2 - Prob. 3COMQCh. 23.2 - Prob. 4COMQCh. 23.3 - Prob. 1COMQCh. 23.3 - 2. Homologous genes
a. are derived from the same...
Ch. 23.3 - Prob. 3COMQCh. 23 - 1. Give the meanings of the following terms:...Ch. 23 - Prob. 2CONQCh. 23 - What is a database? What types of information are...Ch. 23 - Prob. 4CONQCh. 23 - Prob. 5CONQCh. 23 - Prob. 6CONQCh. 23 - Prob. 7CONQCh. 23 - Prob. 9CONQCh. 23 - Which of the following statements uses the term...Ch. 23 - Prob. 1EQCh. 23 - Prob. 2EQCh. 23 - E3. What is meant by the term cluster analysis?...Ch. 23 - Prob. 4EQCh. 23 - 3. Can two-dimensional gel electrophoresis be used...Ch. 23 - Prob. 6EQCh. 23 - 5. Describe the two general types of protein...Ch. 23 - Prob. 8EQCh. 23 - 7. What is a motif? Why is it useful for computer...Ch. 23 - Discuss why it is useful to search a database to...Ch. 23 - Prob. 11EQCh. 23 - E12. Discuss the basis for secondary structure...Ch. 23 - E13. To reliably predict the tertiary structure of...Ch. 23 - Prob. 14EQCh. 23 - In this chapter, we considered a computer program...Ch. 23 - Prob. 16EQCh. 23 - Prob. 17EQCh. 23 - Prob. 18EQCh. 23 - Refer to question 3 in More Genetic TIPS before...Ch. 23 - Prob. 20EQCh. 23 - Prob. 21EQCh. 23 - Prob. 22EQCh. 23 - Prob. 23EQCh. 23 - Prob. 24EQCh. 23 - 1. Let’s suppose you are in charge of organizing...Ch. 23 - Prob. 2QSDC
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