ANATOMY+PHYSIOLOGY >LOOSE<
8th Edition
ISBN: 9781308329826
Author: SALADIN
Publisher: MCG/CREATE
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Chapter 23.1, Problem 2BYGO
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 23 Solutions
ANATOMY+PHYSIOLOGY >LOOSE<
Ch. 23.1 - State at least four functions of the kidneys other...Ch. 23.1 - Prob. 2BYGOCh. 23.1 - Prob. 3BYGOCh. 23.1 - Prob. 1AYLOCh. 23.1 - Prob. 2AYLOCh. 23.1 - Prob. 3AYLOCh. 23.1 - Blood urea nitrogen, azotemia, and uremia, and the...Ch. 23.1 - Prob. 5AYLOCh. 23.2 - Prob. 4BYGOCh. 23.2 - Prob. 5BYGO
Ch. 23.2 - Prob. 6BYGOCh. 23.2 - Prob. 1AYLOCh. 23.2 - Prob. 2AYLOCh. 23.2 - Three layers of tissue that surround and...Ch. 23.2 - Prob. 4AYLOCh. 23.2 - Prob. 5AYLOCh. 23.2 - Prob. 6AYLOCh. 23.2 - Prob. 7AYLOCh. 23.2 - Prob. 8AYLOCh. 23.2 - Prob. 9AYLOCh. 23.2 - Prob. 10AYLOCh. 23.2 - Prob. 11AYLOCh. 23.2 - Prob. 12AYLOCh. 23.3 - Prob. 7BYGOCh. 23.3 - Prob. 8BYGOCh. 23.3 - Prob. 9BYGOCh. 23.3 - Prob. 10BYGOCh. 23.3 - Prob. 1AYLOCh. 23.3 - Prob. 2AYLOCh. 23.3 - Prob. 3AYLOCh. 23.3 - Prob. 4AYLOCh. 23.3 - Prob. 5AYLOCh. 23.3 - Prob. 6AYLOCh. 23.3 - How the myogenic mechanism of renal autoregulation...Ch. 23.3 - Structure and function of the juxtaglomerular...Ch. 23.3 - Prob. 9AYLOCh. 23.3 - Prob. 10AYLOCh. 23.3 - Prob. 11AYLOCh. 23.4 - Prob. 1AYLOCh. 23.4 - Prob. 2AYLOCh. 23.4 - Prob. 3AYLOCh. 23.4 - Prob. 4AYLOCh. 23.4 - Prob. 5AYLOCh. 23.4 - Prob. 6AYLOCh. 23.4 - Prob. 7AYLOCh. 23.4 - The reabsorption of water, Cl-, and glucose by the...Ch. 23.4 - Prob. 12BYGOCh. 23.4 - Prob. 13BYGOCh. 23.5 - Prob. 1AYLOCh. 23.5 - Prob. 2AYLOCh. 23.5 - Prob. 3AYLOCh. 23.5 - Function of the countercurrent multiplier and how...Ch. 23.5 - Prob. 5AYLOCh. 23.5 - Prob. 14BYGOCh. 23.5 - Prob. 15BYGOCh. 23.5 - Prob. 16BYGOCh. 23.6 - Prob. 1AYLOCh. 23.6 - Prob. 2AYLOCh. 23.6 - Prob. 3AYLOCh. 23.6 - Prob. 4AYLOCh. 23.6 - Prob. 5AYLOCh. 23.6 - Prob. 6AYLOCh. 23.6 - Prob. 7AYLOCh. 23.6 - Prob. 8AYLOCh. 23.6 - Prob. 17BYGOCh. 23.6 - Prob. 18BYGOCh. 23.6 - Prob. 19BYGOCh. 23.6 - Prob. 20BYGOCh. 23.7 - Prob. 21BYGOCh. 23.7 - Prob. 22BYGOCh. 23.7 - Prob. 23BYGOCh. 23.7 - The route and mechanism of urine transport from...Ch. 23.7 - Prob. 2AYLOCh. 23.7 - Prob. 3AYLOCh. 23.7 - Anatomy of the male urethra, its three segments,...Ch. 23.7 - The mechanism of the spinal micturition reflex and...Ch. 23.7 - Prob. 6AYLOCh. 23 - Beavers have relatively little need to conserve...Ch. 23 - Prob. 10TYRCh. 23 - Prob. 11TYRCh. 23 - _______ is the ability of a nephron to adjust its...Ch. 23 - The two ureters and the urethra form the...Ch. 23 - The _______ is a group of epithelial cells of the...Ch. 23 - To enter the capsular space, filtrate must pass...Ch. 23 - Glycosuria occurs if the rate of glomerular...Ch. 23 - Prob. 17TYRCh. 23 - Prob. 18TYRCh. 23 - Prob. 19TYRCh. 23 - Prob. 20TYRCh. 23 - Prob. 8BYMVCh. 23 - pyelo-Ch. 23 - Prob. 10BYMVCh. 23 - Prob. 6WWTSCh. 23 - Prob. 7WWTSCh. 23 - The minimum osmolarity of urine is 300 mOsm/L,...Ch. 23 - Prob. 9WWTSCh. 23 - Prob. 10WWTSCh. 23 - Prob. 1BYMVCh. 23 - Prob. 2BYMVCh. 23 - Prob. 3BYMVCh. 23 - Prob. 4BYMVCh. 23 - Prob. 5BYMVCh. 23 - Prob. 6BYMVCh. 23 - Prob. 7BYMVCh. 23 - Prob. 1TYRCh. 23 - The compact ball of capillaries in a nephron is...Ch. 23 - Prob. 3TYRCh. 23 - Prob. 4TYRCh. 23 - Most sodium is reabsorbed from the glomerular...Ch. 23 - Prob. 6TYRCh. 23 - Prob. 7TYRCh. 23 - Prob. 8TYRCh. 23 - Prob. 1WWTSCh. 23 - Prob. 2WWTSCh. 23 - Prob. 3WWTSCh. 23 - Tight junctions prevent material from leaking...Ch. 23 - Prob. 5WWTSCh. 23 - Prob. 1TYCCh. 23 - Prob. 2TYCCh. 23 - Prob. 3TYCCh. 23 - Drugs called renin inhibitors are used to treat...Ch. 23 - Prob. 5TYC
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