EBK ORGANIC CHEMISTRY
8th Edition
ISBN: 8220102744127
Author: Bruice
Publisher: PEARSON
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Chapter 22.13, Problem 28P
Aldolase shows no activity if it is incubated with iodoacetic acid before fructose-1.6-bisphosphate is added to the reaction mixture. What causes this loss of activity?
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Aldolase shows no activity if it is incubated with iodoacetic acid before fructose-1,6-bisphosphate is added to the reaction mixture. What causes this loss of activity?
4. The AGo for the aldolase reaction is 22.8 kJ/mol. When the reaction is run in a hepatocyte cell at
37°C, the concentrations of the intermediates are: fructose 1,6-bisphosphate =1.41 x 103 M,
glyceraldehyde 3-phosphate 2.95 x 10-6 M, and dihydroxyacetone phosphate = 1.62 x 10-5 M.
Calculate the free energy change for this reaction
Is this reaction thermodynamically favorable
5. In the subsequent stages of glycolysis (see figure below): (1) cleavage of FBP to
dihydroxyacetone (DHP) and glyceraldehyde 3-phosphate (G3P) according to FBP →
DHP + G3P is catalyzed by aldolase with K₁ = 104 and (2) the DHP and G3P can
interconvert (DHP → G3P), a process catalyzed by triose phosphate isomerase with K₂ =
0.040. If a 1.75 mM solution of FBP is treated with both of these enzymes (enabling both
of these reactions) at pH 7 and 37°C, find the resulting equilibrium concentration of each
species.
Chapter 22 Solutions
EBK ORGANIC CHEMISTRY
Ch. 22.2 - Compare each of the mechanisms listed here with...Ch. 22.2 - Prob. 3PCh. 22.2 - Prob. 4PCh. 22.3 - a. Draw the mechanism for the following reaction...Ch. 22.5 - Prob. 7PCh. 22.5 - Propose a mechanism for the Co2+ catalyzed...Ch. 22.6 - Prob. 9PCh. 22.7 - Prob. 10PCh. 22.7 - Prob. 12PCh. 22.7 - Prob. 13P
Ch. 22.9 - Which of the following amino acid side chains can...Ch. 22.9 - Which of the following C-terminal peptide bonds is...Ch. 22.9 - Carboxypeptidase A has esterase activity as well...Ch. 22.10 - Arginine and lysine side chains fit into trypsins...Ch. 22.10 - Explain why serine proteases do not catalyze...Ch. 22.11 - If H2 18O is used in the hydrolysis reaction...Ch. 22.11 - Draw the pH-activity profile for an enzyme that...Ch. 22.12 - The pHactivity profile for glucose-6-phosphate...Ch. 22.12 - Prob. 23PCh. 22.13 - Draw the mechanism for the hydroxide ion-catalyzed...Ch. 22.13 - What advantage does the enzyme gain by forming an...Ch. 22.13 - Prob. 26PCh. 22.13 - Prob. 27PCh. 22.13 - Aldolase shows no activity if it is incubated with...Ch. 22 - Which of the following parameters would be...Ch. 22 - Prob. 29PCh. 22 - Prob. 30PCh. 22 - Prob. 31PCh. 22 - Indicate the type of catalysis that is occurring...Ch. 22 - The deuterium kinetic isotope effect (KH2O/KD2O)...Ch. 22 - Prob. 34PCh. 22 - Co2+ catalyzes the hydrolysis of the lactam shown...Ch. 22 - there are two kinds of aldolases. Class I...Ch. 22 - Prob. 37PCh. 22 - The hydrolysis of the ester shown here is...Ch. 22 - Prob. 39PCh. 22 - At pH = 12, the rate of hydrolysis of ester A is...Ch. 22 - 2-Acetoxycyclohexyl tosylate reacts with acetate...Ch. 22 - Proof that an imine was formed between aldolase...Ch. 22 - Prob. 43PCh. 22 - a. Explain why the alkyl halide shown here reacts...Ch. 22 - Triosephosphate isomerase (TIM) catalyzes the...
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- Please draw by hand. Triosephosphate isomerase (TIM) catalyzes the conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate. The enzyme's catalytic groups are Glu 165 and His 95. In the first step of the reaction, these catalytic groups function as a base and an acid catalyst, respectively. Propose a mechanism for the reaction. ОН 2-03Р0 ОН dihydroxyacetone phosphate triosephosphate isomerase 2-03РО. H glyceraldehyde-3-phosphate FYI Glu is glutamic acid and his is histadinearrow_forwardIn the body, during the citric acid cycle the following reaction occurs as the first part of an enzyme mediated process. но OH YYYYYY OH OH H₂O NaBH4 LiAlH4 H₂SO4 and heat K₂Cr2O7 HQ OH What reagent would be used to bring about this reaction in the laboratory? OH 3arrow_forwardAcetolactate synthase transfers the acyl group of pyruvate to alpha-ketobutyrate. This is the first step in the biosynthesis of the amino acid isoleucine. Propose a mechanism for this reaction.arrow_forward
- 2. Using arrow formalism, draw the reaction mechanism for the steps of glycolysis specified below. a. hexokinase: glucose B glucose 6P b. phosphoglucoisomerase: glucose 6P + fructose 6P c. aldolase: fructose 1,6 diphosphate B dihydroxyacetone phosphate + glyceraldehyde 3 phosphate d. triosephosphate isomerase: DHAP + GAParrow_forwardHelparrow_forwardwhat is the structure digested by and how many reducing sugars are present ОН ОН Но OH HO Но HO- OH HO, The trisaccharide pictured could be completely digested (ie into monomers) by a beta-galactosidase and an alpha-glucosidase an alpha-galactosidase and sucrase lactase and sucrase a beta-fructosidase and lactasearrow_forward
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