The mechanism for conversion of carbon dioxide to bicarbonate ion in presence of carbonic anhydrase has to be proposed. Concept introduction: Enzyme is a protein that is a biological catalyst. The reactant in a reaction catalyzed by the enzyme is called substrate. In the enzyme, a pocket like cleft is present called active site where it binds the substrate. Some amino acid chains in the enzyme act as base, acid and nucleophilic catalyst. Many of the enzymes contain metal ions at the active site which acts as catalyst. Acid Catalyst helps in increasing the rate of a particular reaction by the donation of a proton to substrate. Base Catalyst helps in increasing the rate of a particular reaction by the removal of a proton to substrate. Nucleophilic catalysis helps in increasing the rate of a particular reaction by the formation of a covalent bond with substrate Metalloenzyme: An enzyme which has a tightly bound Z n 2 + metal ion

Question

Consider the following biochemical reactions: HO H₂N A B с NH₂ reaction NH₂ ATP ADP enzyme + 2 H₂O enzyme 0 0 FAD FADH₂ ܘܐ ܀ enzyme type of enzyme needed to catalyze this reaction HO dy dy OH In the table below, write the type of enzyme that catalyzes each reaction. Note: be sure to use only the 6 standard enzyme types. NH₂ + 2 NH3 X Ś A B

Answer 1

Question

Chapter 22, Problem 39P

Interpretation Introduction

Interpretation: The mechanism for conversion of carbon dioxide to bicarbonate ion in presence of carbonic anhydrase has to be proposed.

Concept introduction:

Enzyme is a protein that is a biological catalyst. The reactant in a reaction catalyzed by the enzyme is called substrate. In the enzyme, a pocket like cleft is present called active site where it binds the substrate.

Some amino acid chains in the enzyme act as base, acid and nucleophilic catalyst. Many of the enzymes contain metal ions at the active site which acts as catalyst.

Acid Catalyst helps in increasing the rate of a particular reaction by the donation of a proton to substrate.

Base Catalyst helps in increasing the rate of a particular reaction by the removal of a proton to substrate.

Nucleophilic catalysis helps in increasing the rate of a particular reaction by the formation of a covalent bond with substrate

Metalloenzyme: An enzyme which has a tightly bound Zn2+ metal ion

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