Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 21, Problem 2TYU
Summary Introduction
Concept introduction: The Earth was formed before 4.6 billion years from the sun due to the continuous bombardment of huge rocks and ice. When Earth was formed, chemical evolution began, which led to the formation of organic molecules. Organic molecules led to the evolution of first cells and thus life evolved.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 21 Solutions
Biology (MindTap Course List)
Ch. 21.1 - Describe the conditions that scientists think...Ch. 21.1 - Compare the prebiotic soup hypothesis with the...Ch. 21.1 - Prob. 1CCh. 21.1 - How does the ironsulfur world hypothesis differ...Ch. 21.2 - Outline the major steps hypothesized to have...Ch. 21.2 - Explain how the evolution of photosynthetic...Ch. 21.2 - Prob. 5LOCh. 21.2 - Prob. 1CCh. 21.2 - Prob. 2CCh. 21.2 - VISUALIZE Draw a simple sketch illustrating the...
Ch. 21.3 - Briefly describe the distinguishing organisms and...Ch. 21.3 - What is the correct order of appearance in the...Ch. 21.3 - Prob. 2CCh. 21.3 - What is the correct order of appearance in the...Ch. 21 - Energy, the absence of molecular oxygen, chemical...Ch. 21 - Prob. 2TYUCh. 21 - Prob. 3TYUCh. 21 - According to the hypothesis of serial...Ch. 21 - Prob. 5TYUCh. 21 - Prob. 6TYUCh. 21 - Prob. 7TYUCh. 21 - The time of greatest evolutionary diversification...Ch. 21 - Prob. 9TYUCh. 21 - The Age of Reptiles corresponds to the (a)...Ch. 21 - Evidence exists that a catastrophic collision...Ch. 21 - Flowering plants and mammals diversified and...Ch. 21 - Prob. 13TYUCh. 21 - EVOLUTION LINK If you were studying how...Ch. 21 - EVOLUTION LINK If living cells were produced in a...Ch. 21 - EVOLUTION LINK Why did the evolution of...Ch. 21 - INTERPRET DATA Evidence for the oldest...
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