Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 20, Problem 13P
Summary Introduction

To analyze:

The bicoid is characterized as a coordinate, maternal effect gene. Based on this, answer the following:

Determine the phenotype of the progeny produced from the cross between female Drosophila heterozygous for a loss of function bicoid allele and male that is heterozygous for the same allele.

Determine the phenotype of progeny produced from a cross between a female that is homozygous for a loss of function bicoid allele and a wild type male.

Provide an explanation on, if losses of bicoid function in the egg results in lethality during embryogenesis how are females homozygous for bicoid produced. Also determine phenotype of a male homozygous for bicoid loss of function alleles.

Introduction:

Bicoid is an example of a maternal effect gene. The phenotype result from maternal effect gene is identified by the mother genotype and not by the organism phenotype. This is because the egg receives protein and mRNA needed for proper development from the mother for the determination and development of a body axis in an organism. Bicoid is essential, thus, it is also termed as axis determination gene.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

Chapter 20 Solutions

Genetic Analysis: An Integrated Approach (2nd Edition)

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