The tracking of intracellular molecules by super-resolution imaging. Introduction: Super-resolution imaging is the process by which intracellular molecules can be tracked live inside a living system especially a bacterial cell. The process of transport of macromolecules during intracellular events can be easily tracked with the help of specific dyes that can bind to target molecules. The properties of these dyes to absorb and emit light at different wavelengths in visible light can help track many intracellular events. Super-resolution imaging combines the properties of fluorescent color absorbing dyes and emitting dyes to track live intracellular events like cell division, deoxyribose nucleic acid ( DNA) replication. The dye binds to the target molecule and its movement is tracked by the fluorophore emission spectrum. This is based on peak position movements as light from each point appears as a narrow peak. There is an uncertainty of the exact peak position of ten percent yet the tracking of a single protein bound to a fluorophore could be obtained. Another application of this method is the use of green and yellow fluorescent dye to track two key proteins par A and par C involved in bacterial transition between stalked and flagella cells. The movement of the fluorescence across the cell can be tracked because of the different colored dyes confirming the role of the two proteins in the process by live tracking. The emission of light is also not uniform in case fluorophore emits a light.

Question

Want to see the full answer?

Answer 1

Question

Chapter 2, Problem 14RQ

Summary Introduction

To review:

The tracking of intracellular molecules by super-resolution imaging.

Introduction:

Super-resolution imaging is the process by which intracellular molecules can be tracked live inside a living system especially a bacterial cell. The process of transport of macromolecules during intracellular events can be easily tracked with the help of specific dyes that can bind to target molecules. The properties of these dyes to absorb and emit light at different wavelengths in visible light can help track many intracellular events.

Super-resolution imaging combines the properties of fluorescent color absorbing dyes and emitting dyes to track live intracellular events like cell division, deoxyribose nucleic acid (DNA) replication. The dye binds to the target molecule and its movement is tracked by the fluorophore emission spectrum. This is based on peak position movements as light from each point appears as a narrow peak. There is an uncertainty of the exact peak position of ten percent yet the tracking of a single protein bound to a fluorophore could be obtained.

Another application of this method is the use of green and yellow fluorescent dye to track two key proteins par A and par C involved in bacterial transition between stalked and flagella cells. The movement of the fluorescence across the cell can be tracked because of the different colored dyes confirming the role of the two proteins in the process by live tracking. The emission of light is also not uniform in case fluorophore emits a light.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?

You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.

You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb? Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.