MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
MICROBIOLOGY: EVOLV.SCI.-W/ACCESS>CI<
4th Edition
ISBN: 9780393622805
Author: SLONCZEWSKI
Publisher: Norton custom
bartleby

Concept explainers

Question
Book Icon
Chapter 2.5, Problem 2TQ
Summary Introduction

To review:

The comparison of fluorescence and darkfield microscopy. Also, mention similar advantages of these techniques and the variations in the common advantage.

Introduction:

Microscopy started with the basic light microscope and with the passage of time, it has developed into various advanced types. The different types of advanced microscopy techniques are associated with the visualization of a range of structures. The resolution and the detection power of each type of microscopic technique may vary in many aspects.

Fluorescence microscopy is used to detect intracellular events that occur inside a bacterial cell. For example, replication with the help of colored dyes that can absorb and emit light of specific wavelengths. Darkfield microscopy is used for distinguishing certain extracellular features of bacteria like flagella by reducing the intensity of incident light.

The specimens or objects that have dimensions less than that of the wavelength of light can be visualized by both fluorescence and dark-field microscopy. Both of these techniques are able to detect such objects/specimens but these techniques are not able to resolve them. The differences between fluorescence and dark-field microscopy are mentioned below:

Blurred answer
Students have asked these similar questions
Sample excuse letter in school class for the reasons of headaches and dysmenorrhea caused by menstrual cycle
How do the muscles on the foot work to balance on an ice skate, specifically the triangle of balance and how does it change when balancing on an ice skate? (Refer to anatomy, be specific)
Which of the following is NOT an example of passive immunization? A. Administration of tetanus toxoid B. Administration of hepatitis B immunoglobulin C. Administration of rabies immunoglobulin D. Transfer of antibodies via plasma therapy
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
Text book image
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage
Text book image
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage
Text book image
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Text book image
Concepts of Biology
Biology
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:OpenStax College
Text book image
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage