Chapter 19.3, Problem 31AQP

Summary Introduction

To explain:

Compare and contrast the use PCR and gene cloning for amplifying DNA fragments.

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA; it is of many types like, real time PCR, nested PCR, and semi-nested PCR. PCR is having three main steps; Denaturing, Annealing, and Extension. PCR reaction contains template DNA, Primers, Deoxynucleotide triphosphates, DNA polymerase, Mg ${}^{2+}$ ions, and buffer.

Gene cloning is a molecular technique, in which we make copies of DNA by inserting DNA into the vector like plasmid. It is done by the help of restriction enzyme, DNA ligases and vector.

These both methods are used to make copies of DNA. DNA amplification is the process in which by using gene cloning or PCR make copies of DNA.

Summary Introduction

To determine:

Advantages and disadvantage of gene cloning and PCR

Introduction:

PCR (polymerase chain reaction) is a technique used in molecular biology to amplify a single copy or few copies of segment of DNA to synthesize the new strand of DNA. PCR is used to make copies of DNA.

Gene cloning is also a molecular technique used in molecular biology to amplify the DNA segment by inserting in the vector like plasmid.