Chapter 19.2, Problem 26AQP

Summary Introduction

To determine:

How often, an average type II restriction enzyme cut a DNA molecule if the recognition sequence for the enzyme had $5$ bp?

Introduction:

Restriction enzymes are the proteins, which cut the DNA at specific restriction site, also called by the name restriction endonucleases. Restriction enzyme is of four types, depend on their specificity they are distributed in the type. Four types of restriction enzymes are type I, type II, type III and type IV restriction enzyme. Type II restriction enzyme is mostly used in molecular genetics, because of their specificity , they cut directly on the restriction site and they only required Mg $2^{+}$ ions as cofactor.

Summary Introduction

To determine:

How often, an average type II restriction enzyme cut a DNA molecule if the recognition sequence for the enzyme had $8$ bp?

Introduction:

Type II restriction enzyme is more specific type of restriction enzyme, it cut DNA molecule at the specific site. All restriction enzymes are obtained from the bacteria.