Chapter 19.5, Problem 8CC

Summary Introduction

Introduction:

Sanger’s method or dideoxy sequencing is a method of gene sequencing. It is also known by the name of chain termination method. This method required a single stranded DNA template, DNA primer, DNA polymerase, dNTPs (Deoxynucleoside Triphosphates) and ddNTPs (Di-Deoxynucleotide Triphosphates).

Single stranded DNA template is used to synthesize complimentary copy of DNA with the help of DNA polymerase in the presence of nucleotides. DNA polymerase utilizes ddNTPs as nucleotide, and it gets included into the growing chain, but chain elongation stops at ddNTPs due to absence of $3\text{'}$-OH group. In this, 4 different ddNTPs (A, T, G, and C) are used and they take 4 separate reactions.