Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 19, Problem 8P
Outline the steps required for a gene originally present in the endosymbiont genome to be transferred to the nuclear genome and be expressed, and for its product to be targeted back to the organelle of origin.
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The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid:
a. Between which two stages of the Neurospora life cycle do most mitotic cell divisions occur?
b. Neurospora has an arginine amino acid synthesis pathway shown below:
Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning.
c. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain:
i) What percentage of the progeny will grow on ornithine?
ii) What percentage on citrulline?
iii) What percentage on arginine?
Show your work for i), ii) and iii). [Can be answered in less…
Expression of a gene, in terms of greater accumulation of the protein it encodes, could
be increased by.
A) increasing transcription of that gene.
B) inhibiting proteases that break down the protein it encodes
C) increasing the half life of its MRNA transcript
D) A and C
E) A, B and C
You are interested in studying a novel gene that appears to be involved in cancer. There is no information about the function of this gene. What would you do to obtain the cDNA for this gene? How would you express this gene and what expression systems might you utilize to study its function and why? How would determine the subcellular localization of this gene in eukaryotic cells? What are alternative methods in case one doesn't work? How would you purify and determine the 3-dimensional structure of this protein?
Chapter 19 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 19 - 17.1 Reciprocal crosses of experimental animals or...Ch. 19 - 17.2 How are some of the characteristics of the...Ch. 19 - 17.3 The human mitochondrial genome encodes...Ch. 19 - What is the evidence that transfer of DNA from the...Ch. 19 - Draw a graph depicting the relative amounts of...Ch. 19 - Prob. 6PCh. 19 - 17.7 What is the evidence that the ancient...Ch. 19 - 17.8 Outline the steps required for a gene...Ch. 19 - 17.9 Consider the phylogenetic tree presented in...Ch. 19 - Most large protein complexes in mitochondria and...
Ch. 19 - What insights have analyses of human mitochondrial...Ch. 19 - You are a genetic counselor, and several members...Ch. 19 - A mutation in Arabidopsis immutans results in the...Ch. 19 - What type or types of inheritance are consistent...Ch. 19 - You have isolated (1) a streptomycin-resistant...Ch. 19 - You have isolated two petite mutants, pet1 and...Ch. 19 - 17.15 Consider this human pedigree for a vision...Ch. 19 - A 50- year - old man has been diagnosed with MELAS...Ch. 19 - 17.17 The first person in a family to exhibit...Ch. 19 - Prob. 20PCh. 19 - Prob. 21PCh. 19 - 17.19 What is the most likely mode of inheritance...Ch. 19 - 17.20 In , the Russian Tsar Nicholaswas deposed,...Ch. 19 - 17.21 The dodo bird (Raphus cucullatus) lived on...Ch. 19 - Cytoplasmic male sterility (CMS) in plants has...Ch. 19 - 17.23 Wolves and coyotes can interbreed in...Ch. 19 - Prob. 27PCh. 19 - Prob. 28P
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