Microbiology with Diseases by Body System & Modified MasteringMicrobiology with Pearson eText -- ValuePack Access Card -- for Microbiology with Diseases by Body System Package
1st Edition
ISBN: 9780133857122
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 19, Problem 3MTF
Summary Introduction
The surface cleansers are the type of cleansing agent consists of chemicals. Example for the surface cleanser is Dettol. The main role of antibacterial surface cleanser is killing the bacteria, flu virus, and E.coli.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 19 Solutions
Microbiology with Diseases by Body System & Modified MasteringMicrobiology with Pearson eText -- ValuePack Access Card -- for Microbiology with Diseases by Body System Package
Ch. 19 - Prob. 1TMWCh. 19 - Prob. 2TMWCh. 19 - Buruli Ulcer Jacques liked living in the...Ch. 19 - Prob. 3TMWCh. 19 - A Painful Rash A mother brings her three-year-old...Ch. 19 - A Child with Warts Ten-year-old Rudy has several...Ch. 19 - Grandfathers Shingles The Davises were excited...Ch. 19 - Prob. 4TMWCh. 19 - Prob. 4CCSCh. 19 - Prob. 5TMW
Ch. 19 - Diagnosis in the Desert In the 1970s,...Ch. 19 - Prob. 2EDCSCh. 19 - The epidermis ____. a. has an intricate network of...Ch. 19 - Prob. 2MCCh. 19 - Prob. 3MCCh. 19 - Prob. 4MCCh. 19 - Prob. 5MCCh. 19 - Prob. 6MCCh. 19 - Prob. 7MCCh. 19 - Prob. 8MCCh. 19 - Prob. 9MCCh. 19 - Prob. 10MCCh. 19 - Prob. 11MCCh. 19 - Prob. 12MCCh. 19 - Prob. 13MCCh. 19 - Prob. 14MCCh. 19 - Prob. 15MCCh. 19 - Prob. 1MCh. 19 - Prob. 1FIBCh. 19 - Prob. 2FIBCh. 19 - Prob. 3FIBCh. 19 - Prob. 4FIBCh. 19 - Prob. 5FIBCh. 19 - Prob. 6FIBCh. 19 - Prob. 7FIBCh. 19 - Prob. 8FIBCh. 19 - Prob. 9FIBCh. 19 - Most pimples result from infection by ______ not...Ch. 19 - Prob. 1MTFCh. 19 - Prob. 2MTFCh. 19 - Prob. 3MTFCh. 19 - Prob. 4MTFCh. 19 - Prob. 5MTFCh. 19 - Prob. 1SACh. 19 - Prob. 2SACh. 19 - Considering that Pseudomonas aeruginosa and...Ch. 19 - Prob. 4SACh. 19 - Prob. 5SACh. 19 - Describe four enzymes produced by Staphylococcus,...Ch. 19 - Prob. 7SACh. 19 - Prob. 8SACh. 19 - Prob. 9SACh. 19 - Prob. 10SACh. 19 - Label each lesion seen in poxvirus infections.Ch. 19 - Prob. 2VICh. 19 - Prob. 3VICh. 19 - Prob. 1CTCh. 19 - Why is it more difficult to rid a community of a...Ch. 19 - Prob. 3CTCh. 19 - Prob. 4CTCh. 19 - Prob. 5CTCh. 19 - Most DNA viruses replicate within the nucleus of a...Ch. 19 - Prob. 7CTCh. 19 - Prob. 8CTCh. 19 - Prob. 9CTCh. 19 - Prob. 10CTCh. 19 - Prob. 11CTCh. 19 - Why do impetigo and erysipelas occur more commonly...Ch. 19 - Prob. 13CTCh. 19 - Prob. 14CTCh. 19 - Prob. 1CM
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