Whether the gene GAL4 or GAL80 genes can be considered as potential candidates for isolation of mutations that allow the constitutive expression of the target genes along with the characteristic of protein that disrupts the mutation. Introduction: Yeast genes have cis-acting elements upstream of their promoters and are similar to enhancers, which are called upstream activating sequences (UASs). UAS G regulates the several target genes involved in the galactose utilization.

Question

19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

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Question

Chapter 17, Problem 8P

Summary Introduction

To determine:

Whether the gene GAL4 or GAL80 genes can be considered as potential candidates for isolation of mutations that allow the constitutive expression of the target genes along with the characteristic of protein that disrupts the mutation.

Introduction:

Yeast genes have cis-acting elements upstream of their promoters and are similar to enhancers, which are called upstream activating sequences (UASs). UAS_G regulates the several target genes involved in the galactose utilization.

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