Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 17, Problem 15MC
Summary Introduction
Introduction:
The blood is analyzed for the existence of a specific antibody and antigens by a method termed as serology. There are wide range of serological tests available to recognize the antigens or antibodies in the blood. Using tagged or labelled antibody to identify an antigen is an altered form of serological testing method, for example ELISA, fluorescent antibody tests (direct and indirect), and immunoblotting tests. ELISA uses enzyme labelled anti-antibody as a reagent that attaches to the antibody (binds to antigen of choice).
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 17 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 17 - Prob. 1TMWCh. 17 - A diagnostician used an ELISA to show that a...Ch. 17 - To obtain immediate immunity against tetanus, a...Ch. 17 - Which of the following vaccine types is commonly...Ch. 17 - Prob. 3MCCh. 17 - When antigen and antibodies combine, maximal...Ch. 17 - An anti-antibody is used when _________. a. an...Ch. 17 - Prob. 6MCCh. 17 - Prob. 7MCCh. 17 - Prob. 8MC
Ch. 17 - Prob. 9MCCh. 17 - Prob. 10MCCh. 17 - Prob. 11MCCh. 17 - An antiserum is ________. a. an anti-antibody b....Ch. 17 - Prob. 13MCCh. 17 - Prob. 14MCCh. 17 - Prob. 15MCCh. 17 - Prob. 1MTFCh. 17 - Prob. 2MTFCh. 17 - Prob. 3MTFCh. 17 - Modified True/False 4. _________________ ELISA is...Ch. 17 - Prob. 5MTFCh. 17 - Match the characteristic in the first column with...Ch. 17 - Identify the chemicals represented by this artists...Ch. 17 - Prob. 2VICh. 17 - Compare and contrast the Chinese practice of...Ch. 17 - What are the advantages and disadvantages of...Ch. 17 - Compare the advantages and disadvantages of...Ch. 17 - How does precipitation differ from agglutination?Ch. 17 - Explain how a pregnancy test works at the...Ch. 17 - Compare and contrast herd immunity and contact...Ch. 17 - How does nephelometry differ from turbidimetry?Ch. 17 - Is it ethical to approve the use of a vaccine that...Ch. 17 - Which is worse: to use a diagnostic test for HIV...Ch. 17 - Discuss the importance of costs and technical...Ch. 17 - What bodily fluids, in addition to blood serum,...Ch. 17 - Why might a serological test give a false positive...Ch. 17 - Some researchers want to distinguish B cells from...Ch. 17 - Describe three ways by which genetic recombinant...Ch. 17 - How does a toxoid vaccine differ from an...Ch. 17 - Explain why many health organizations promote...Ch. 17 - Contrast a hemagglutination test with a viral...Ch. 17 - Prob. 11CTCh. 17 - Draw a picture showing, at both the molecular and...Ch. 17 - Using the following terms, fill in the following...
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