Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 17, Problem 2VI
Summary Introduction
To answer:
Which patients are most likely uninfected using the immunoblot results for a particular antigen?
Introduction:
A western blot is also termed as immunoblot. It is a testing method used to identify a particular protein molecule, for example, an antibody (complex mixture). General practitioner use western blot in order to confirm the occurrence of HIV antibodies or proteins (blood serum) against the bacterium that cause Lyme disease in human.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay).
1.In the "ELISA sandwich," what is the "meat"?
2.Both patients A and B had positive results. Why are these two patients' outcomes so different?
3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?
The attached image has the following Newick format:
((HIV-1 retropepsin:0.67441,(HIV-2 retropepsin:0.40370, Simian immunodeficiency:0.40370):0.27071):0.963537,(Bovine immunodeficiency:1.34405,Feline immunodeficiency:1.34405):0.295328);
Examine the branch lengths. What do they tell you about the evolutionary time between the branch points?• Which branchings are more ancient and which are more recent?• What would you conclude about these immunodeficiency virus proteases, based on this small analysis?
A student completed a plate and liquid lysate followed by a plaque assay. The volume harvested for the plate lysate was 5.5mL and had a titre of 1.8x10^10 PFU/mL. The volume harvested for the liquid lysate was 9.5mL and had a titre of 1.1x10^10 PFU/mL. Which of these lysates had the highest viral yield?
Liquid lysate had the highest yield
Plate lysate had the highest yield
They both have the same yield
Chapter 17 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 17 - Prob. 1TMWCh. 17 - A diagnostician used an ELISA to show that a...Ch. 17 - To obtain immediate immunity against tetanus, a...Ch. 17 - Which of the following vaccine types is commonly...Ch. 17 - Prob. 3MCCh. 17 - When antigen and antibodies combine, maximal...Ch. 17 - An anti-antibody is used when _________. a. an...Ch. 17 - Prob. 6MCCh. 17 - Prob. 7MCCh. 17 - Prob. 8MC
Ch. 17 - Prob. 9MCCh. 17 - Prob. 10MCCh. 17 - Prob. 11MCCh. 17 - An antiserum is ________. a. an anti-antibody b....Ch. 17 - Prob. 13MCCh. 17 - Prob. 14MCCh. 17 - Prob. 15MCCh. 17 - Prob. 1MTFCh. 17 - Prob. 2MTFCh. 17 - Prob. 3MTFCh. 17 - Modified True/False 4. _________________ ELISA is...Ch. 17 - Prob. 5MTFCh. 17 - Match the characteristic in the first column with...Ch. 17 - Identify the chemicals represented by this artists...Ch. 17 - Prob. 2VICh. 17 - Compare and contrast the Chinese practice of...Ch. 17 - What are the advantages and disadvantages of...Ch. 17 - Compare the advantages and disadvantages of...Ch. 17 - How does precipitation differ from agglutination?Ch. 17 - Explain how a pregnancy test works at the...Ch. 17 - Compare and contrast herd immunity and contact...Ch. 17 - How does nephelometry differ from turbidimetry?Ch. 17 - Is it ethical to approve the use of a vaccine that...Ch. 17 - Which is worse: to use a diagnostic test for HIV...Ch. 17 - Discuss the importance of costs and technical...Ch. 17 - What bodily fluids, in addition to blood serum,...Ch. 17 - Why might a serological test give a false positive...Ch. 17 - Some researchers want to distinguish B cells from...Ch. 17 - Describe three ways by which genetic recombinant...Ch. 17 - How does a toxoid vaccine differ from an...Ch. 17 - Explain why many health organizations promote...Ch. 17 - Contrast a hemagglutination test with a viral...Ch. 17 - Prob. 11CTCh. 17 - Draw a picture showing, at both the molecular and...Ch. 17 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Anti-U antibodies can be produced by which of the following genotypes? Please select the single best answer M-N- S+s+ S+s- S-s-arrow_forwardwhy do we have to do a western blot confirmatory testing after doing an ELISA screening test for HIV?arrow_forwardYou currently work in a BLS 2 lab with organisms that are classified as BSL 1 or 2. Your project manager gives you your next assignment, as you look down the list of organisms that you will be performing susceptibility tests, your eyes stop on Bacillus subtilis and Bacillus anthrax. 1) What gave you pause about these bacteria? 2) What conversation do you need to have with your project manager? no references, just homeworkarrow_forward
- In figure 3, what do the letters C, S, P, and m indicate in panel A? I need help finding the answer in the article link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/arrow_forwardA technologist failed to notice that the centrifuge had not properly centrifuged the test tubes prepared for antibody identification. The time of centrifugation was 15 seconds instead of 30 seconds. What would be the potential error in the interpretation of this test? can please any one provide me ans?arrow_forwardA 3-year-old girl with a fever is sent to the hospital and is found to have a Pseudomonas sp. infection. Her platelet count was 60 x109/L, her haemoglobin value was 74 g/L, her white blood cell count was 1x109/L, and blast cells were seen on a peripheral blood film. Ninety percent of the cells in the bone marrow were blasts, according to a smear. Upon immunophenotyping these blasts using flow cytometry, positive phenotypes for CD10, CD19, (partial)TdT, and CD34 were observed.arrow_forward
- If a patient was nonadherent to HIV drugs, in this case, what does nonadherent mean?arrow_forwardWhy were RH-negative red blood cells used for the intrauterine transfusion?arrow_forwardidentify the different cells in the blood smear image by their phenotypes and state their primary function in the table belowarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
