Principles of Human Physiology Plus Mastering A&P with Pearson eText - Access Card Package (6th Edition)
6th Edition
ISBN: 9780134169040
Author: Cindy L. Stanfield
Publisher: PEARSON
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Chapter 16.3, Problem 16.2.1QC
Summary Introduction
To write:
During inspiration, the atmospheric pressure or intra-alveolar pressure, which one has the greater value.
Introduction:
In the human body, the alveolar and intrapleural pressures are dependent on certain physical features of the lung. However, during the inspiration, the ability to breathe and air leaves the lungs during expiration is dependent on the atmospheric air pressure and the air pressure present within the lungs
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 16 Solutions
Principles of Human Physiology Plus Mastering A&P with Pearson eText - Access Card Package (6th Edition)
Ch. 16.2 - Prob. 1CTQCh. 16.2 - What are the primary causes of the two types of...Ch. 16.2 - Describe the behavioral modifications and surgical...Ch. 16.2 - Prob. 16.1.1QCCh. 16.2 - Prob. 16.1.2QCCh. 16.2 - Prob. 16.1.3QCCh. 16.3 - Prob. 16.2.1QCCh. 16.3 - Prob. 16.2.2QCCh. 16.3 - Prob. 16.2.3QCCh. 16.4 - Prob. 1CTQ
Ch. 16.4 - Prob. 2CTQCh. 16.4 - Prob. 3CTQCh. 16.4 -
If scarring of the lung resulted from some...Ch. 16.4 - Prob. 16.3.2QCCh. 16.4 - Prob. 16.3.3QCCh. 16.5 - Prob. 16.4.1QCCh. 16.5 - Following a maximum inspiration, a person expires...Ch. 16.5 - Prob. 16.4.3QCCh. 16 - Prob. 1ECh. 16 - Prob. 2ECh. 16 - Prob. 3ECh. 16 - Prob. 4ECh. 16 - Prob. 5ECh. 16 - Prob. 6ECh. 16 - Prob. 7ECh. 16 - Prob. 8ECh. 16 - Prob. 9ECh. 16 - Prob. 10ECh. 16 - Prob. 11ECh. 16 - Prob. 12ECh. 16 - Prob. 13ECh. 16 - Prob. 14ECh. 16 - Prob. 15ECh. 16 - Prob. 16ECh. 16 - Prob. 17ECh. 16 - Prob. 18ECh. 16 - Prob. 19ECh. 16 - The (internal/external) intercostals are muscles...Ch. 16 - Describe the various ways in which the structure...Ch. 16 - Prob. 22ECh. 16 - Prob. 23ECh. 16 - Prob. 24ECh. 16 - Prob. 25ECh. 16 - Prob. 26ECh. 16 - Prob. 27ECh. 16 - Prob. 28ECh. 16 - Prob. 29E
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