Principles of Human Physiology Plus Mastering A&P with Pearson eText - Access Card Package (6th Edition)
6th Edition
ISBN: 9780134169040
Author: Cindy L. Stanfield
Publisher: PEARSON
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Chapter 16.4, Problem 2CTQ
Summary Introduction
To write:
The process that leads to loss of elastic fiber in the lungs.
Introduction:
Emphysema is the condition which is caused when the walls of the air sacs get destroyed in the human body. In this condition, the inner walls of the air sacs weaken and burst, which leads to the formation of one large space to hold air instead of many small ones.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 16 Solutions
Principles of Human Physiology Plus Mastering A&P with Pearson eText - Access Card Package (6th Edition)
Ch. 16.2 - Prob. 1CTQCh. 16.2 - What are the primary causes of the two types of...Ch. 16.2 - Describe the behavioral modifications and surgical...Ch. 16.2 - Prob. 16.1.1QCCh. 16.2 - Prob. 16.1.2QCCh. 16.2 - Prob. 16.1.3QCCh. 16.3 - Prob. 16.2.1QCCh. 16.3 - Prob. 16.2.2QCCh. 16.3 - Prob. 16.2.3QCCh. 16.4 - Prob. 1CTQ
Ch. 16.4 - Prob. 2CTQCh. 16.4 - Prob. 3CTQCh. 16.4 -
If scarring of the lung resulted from some...Ch. 16.4 - Prob. 16.3.2QCCh. 16.4 - Prob. 16.3.3QCCh. 16.5 - Prob. 16.4.1QCCh. 16.5 - Following a maximum inspiration, a person expires...Ch. 16.5 - Prob. 16.4.3QCCh. 16 - Prob. 1ECh. 16 - Prob. 2ECh. 16 - Prob. 3ECh. 16 - Prob. 4ECh. 16 - Prob. 5ECh. 16 - Prob. 6ECh. 16 - Prob. 7ECh. 16 - Prob. 8ECh. 16 - Prob. 9ECh. 16 - Prob. 10ECh. 16 - Prob. 11ECh. 16 - Prob. 12ECh. 16 - Prob. 13ECh. 16 - Prob. 14ECh. 16 - Prob. 15ECh. 16 - Prob. 16ECh. 16 - Prob. 17ECh. 16 - Prob. 18ECh. 16 - Prob. 19ECh. 16 - The (internal/external) intercostals are muscles...Ch. 16 - Describe the various ways in which the structure...Ch. 16 - Prob. 22ECh. 16 - Prob. 23ECh. 16 - Prob. 24ECh. 16 - Prob. 25ECh. 16 - Prob. 26ECh. 16 - Prob. 27ECh. 16 - Prob. 28ECh. 16 - Prob. 29E
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