Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Chapter 15, Problem 11TYU
EVOLUTION CONNECTION Crossing over is thought to be evolutionarily advantageous because it continually shuffles genetic alleles into novel combinations. Until recently, it was thought that the genes on the Y chromosome might degenerate because they lack homologous genes on the X chromosome with which to pair up prior to crossing over. However, when the Y chromosome was sequenced, eight large regions were found to be internally homologous to each other, and quite a few of the 78 genes represent duplicates. (Y chromosome researcher David Page has called it a "hall of mirrors") Explain what might be a benefit of these regions.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 15 Solutions
Campbell Biology: Custom Edition
Ch. 15.1 - Which one of Mendel's laws describes the...Ch. 15.1 - MAKE CONNECTIONS Review the description of...Ch. 15.1 - WHAT IF? Propose a possible reason that the first...Ch. 15.2 - A white-eyed female Drosophila is mated with a...Ch. 15.2 - Neither Tim nor Rhoda has Duchenne muscular...Ch. 15.2 - MAKE CONNECTIONS Consider what you learned about...Ch. 15.3 - When two genes are located on the same chromosome,...Ch. 15.3 - VISUAL SKILLS For each type of offspring of the...Ch. 15.3 - Prob. 3CCCh. 15.4 - Prob. 1CC
Ch. 15.4 - Prob. 2CCCh. 15.4 - Prob. 3CCCh. 15.5 - Gene dosagethe number of copies of a gene that are...Ch. 15.5 - Reciprocal crosses between two primrose varieties,...Ch. 15.5 - WHAT IF? Mitochondrial genes are critical to the...Ch. 15 - What characteristic of the sex chromosomes allowed...Ch. 15 - Why are males affected by X-Iinked disorders much...Ch. 15 - Why are specific alleles of two distant genes more...Ch. 15 - Prob. 15.4CRCh. 15 - Explain how genomic imprinting and inheritance of...Ch. 15 - A man with hemophilia (a recessive, sex-linked...Ch. 15 - Pseudohypertrophic muscular dystrophy is an...Ch. 15 - A wild-type fruit fly (heterozygous for gray body...Ch. 15 - A planet is inhabited by creatures that reproduce...Ch. 15 - Using the information from problem 4, scientists...Ch. 15 - A wild-type fruit fly (heterozygous for gray body...Ch. 15 - Assume that genes, A and B are on the same...Ch. 15 - Two genes of a flower, one Controlling blue (B)...Ch. 15 - You design Drosophila crosses to provide...Ch. 15 - Banana plants, which are triploid, are seedless...Ch. 15 - EVOLUTION CONNECTION Crossing over is thought to...Ch. 15 - SCIENTIFIC INQUIRY DRAW IT Assume you are mapping...Ch. 15 - WRITE ABOUT A THEME: INFORMATION The continuity of...Ch. 15 - SYNTHESIZE YOUR KNOWLEDGE Butter flies have an X-Y...
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