Campbell Biology: Custom Edition
Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Chapter 15.5, Problem 3CC

WHAT IF? Ø Mitochondrial genes are critical to the energy metabolism of cells, but mitochondrial disorders caused by mutations in these genes are generally not lethal. Why not?

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1A. Name and draw diagrammatically the series of mitochondrial electron transfer catalysts, starting with the oxidation of NADH and succinate and ending with the reduction of O₂. B. Indicate the sites and stoichiometry (per 2e) at which protons are translocated from the matrix to the intermembrane space. C. Indicate which complexes are inhibited by: amytal, antimycin A, azide (N3), cyanide (CN), carbon monoxide (CO), and rotenone.
During the FADH2 electron transport chain,      the protons are pumped from the mitochondrial matrix to the intermembrane space through complexes CoQ/III, and IV     the protons are pumped from the mitochondrial matrix to the intermembrane space through complexes I, CoQ/III, and IV     the protons are pumped from the mitochondrial matrix to the intermembrane space through complexes I, CoQ/II, and IV     the protons are pumped from the mitochondrial matrix to the outer mitochondrial membrane through complexes I, III, and IV
1. Name and draw diagrammatically the series of mitochondrial electron transfer catalysts, starting with the oxidation of NADH and succinate and ending with the reduction of O2. 2. Indicate the sites and stoichiometry (per 2e) at which protons are translocated from the matrix to the intermembrane space. 3. Indicate which complexes are inhibited by: amytal, antimycin A, azide (N3 ), cyanide (CN), carbon monoxide (CO), and rotenone. 4. Describe the effects of (1) oligomycin and (2) uncouplers of oxidative phosphorylation, e.g., dinitrophenol (DNP), carbonyl cyanide-p- trifluoromethoxyphenylhydrazone (FCCP), on respiration and ATP synthesis when added to a suspension of mitochondria with excess malate, ADP, and inorganic phosphate (Pi).

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Campbell Biology: Custom Edition

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