EBK BIOLOGY
11th Edition
ISBN: 8220106820636
Author: Martin
Publisher: CENGAGE L
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Chapter 14.2, Problem 1C
Summary Introduction
To explain: The function of each of the parts of the lac operon (promoter, operator, CAP binding site and structural genes).
Introduction: Operons are a set of genes containing a promoter and one or more operators that helps to control the transcription process. Each operon is made of structural genes, promoter and operator. Inducible operons are turned on by the presence of the substrate. Lac operon is an inducible operon.
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What is the function of each of the parts of the lac operon (promoter, operator, CAP binding site, and structural genes)?
a) State the functions of the subunits of RNA polymerase and describe transcription initiation.
b)Draw a diagram to illustrate the lac operon and explain how it functions in the presence of, i) glucose and ii) lactose in the culture medium
What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case!
A) Glucose is absent and lactose is present
B) Glucose is present and lactose is present
C) Glucose is present and lactose is absent
Chapter 14 Solutions
EBK BIOLOGY
Ch. 14.1 - Explain why bacterial and eukaryotic cells have...Ch. 14.1 - Prob. 1CCh. 14.1 - Prob. 2CCh. 14.2 - Prob. 2LOCh. 14.2 - Distinguish among inducible, repressible, and...Ch. 14.2 - Differentiate between positive and negative...Ch. 14.2 - Prob. 5LOCh. 14.2 - Prob. 1CCh. 14.2 - What structural features does the trp operon share...Ch. 14.2 - Prob. 3C
Ch. 14.2 - Prob. 4CCh. 14.3 - Prob. 6LOCh. 14.3 - Give examples of some of the ways eukaryotic...Ch. 14.3 - Prob. 8LOCh. 14.3 - Prob. 9LOCh. 14.3 - Prob. 10LOCh. 14.3 - Prob. 1CCh. 14.3 - Prob. 2CCh. 14.3 - Prob. 3CCh. 14.3 - Prob. 4CCh. 14.3 - Prob. 5CCh. 14 - The regulation of most bacterial genes occurs at...Ch. 14 - Prob. 2TYUCh. 14 - Prob. 3TYUCh. 14 - Prob. 4TYUCh. 14 - Inactive genes tend to be found in (a) highly...Ch. 14 - Prob. 6TYUCh. 14 - Which of the following is characteristic of genes...Ch. 14 - Through alternative splicing, eukaryotes (a)...Ch. 14 - A mutation that inactivates the repressor gene of...Ch. 14 - Which of the following is an example of positive...Ch. 14 - Prob. 11TYUCh. 14 - PREDICT Compare the types of bacterial genes...Ch. 14 - INTERPRET DATA Develop a simple hypothesis that...Ch. 14 - Prob. 14TYUCh. 14 - Prob. 15TYUCh. 14 - EVOLUTION LINK Suggest why evolution resulted in...Ch. 14 - Prob. 17TYU
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- What are the main elements of the lac operon and their functions?arrow_forwardIf a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would you expect? A) The three genes of the lac operon will be expressed normally. B) The repressor will not be able to bind to the operon. C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated. D) LacZ will not be transcribed and Beta galactosidase will not be produced.arrow_forwardWhat is lac operon? Draw and/or identify the status of the lac operon in a given set of environmental conditions and/or cell mutations.arrow_forward
- What is the phenotype of an E. coli strain with a mutation in the lac operator that allows the lac repressor to irreversibly bind the operator? Assume glucose is absent. 1S A) O Transcription of the operon is very high whether lactose is present or not. B) O Transcription of the operon is very low whether lactose is present or not. C) O Transcription of the operon is high in the presence of lactose, and low in its absence. D) O Transcription of the operon is low in the presence of lactose, and high in its absence.arrow_forwardThe attenuation mechanism that helps block expression of the tryptophan operon requires all of the following, except A) O MRNA transcript of the trp leader sequence B) Oaribosome stalled at the stop codon of the trp leader transcript C) O formation of an attenuator loop D) O low intracellular levels of tryptophanarrow_forwardA mutation prevents the catabolite activator protein (CAP) from binding to the promoter in the lac operon. What will the effect of this mutation be on the transcription of the operon?arrow_forward
- If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon? a) There is a mutation in the repressor protein b) There is a mutation in the catabolite activator protein c) There is a mutation in the promoter region d) There is a mutation in the luxI proteinarrow_forwardThe transcription of many bacterial genes relies on functional groups called operons, such as the tryptophan operon. What is an operon?arrow_forwardDescribe the structure of the Lac operon. How is it turned on? How is it turned off?arrow_forward
- Predict the effect on the activity of the lac operon of a mutation that disrupts the function of: a) the cap gene, which encodes the CAP protein. b) the CAP-binding site.arrow_forwardOne way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressorarrow_forwardDescribe the organization of the lac operon.arrow_forward
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