1 SEM CARDLESS ACC W/RAVEN TEXT
12th Edition
ISBN: 9781265321062
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 14, Problem 6U
Summary Introduction
Introduction:
Almost all the reactions of the body are controlled or governed by one other enzyme. Like for example if we take the case of digestion there are many enzymes that are involved in the process of digestion so is the case with the replication of DNA. In the process of replication of DNA there are certain enzymes that are needed for the process to be carried out. For example: DNA gyrase, DNA helicase, DNA polymerase and so on.
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Which of the following statements is FALSE regarding the molecular mechanism for DNA polymerases?
A.
The active site contains 2 divalent metal ions
B.
A single stranded DNA template is required
C.
The enzyme can only attach a new deoxynucleotide to the 5’ end of a growing chain
D.
The 3’OH on the deoyxyribose ring attacks a phosphate of a dNTP to produce a new phosophodiester bond
E.
None of the above (all are true statements)
Which statement about Okazaki fragments is true?
Select one:
a. DNA polymerase doesn’t need a primer to build these fragments
b. They act as a primer that initiates DNA replication.
c. They correct errors made during earlier phases of DNA replication.
d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork.
e. They prevent the ends of chromosomes from shortening with every replication.
The function of DNA ligase is to:
a. Catalyze formation of phosphodiester bonds between adjacent nucleotides
b. Catalyze formation of hydrogen bonds between adjacent nucleotides
c. Keep single strands of DNA apart during replication
d. Facilitate base pairing between single stranded molecules in DNA
e. Both a. and d. are correct
Chapter 14 Solutions
1 SEM CARDLESS ACC W/RAVEN TEXT
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- a. unwinds the DNA helix b. stabilizes and stops the two strands from annealing (rebinding with each other) c. recoils the DNA d. cleaves both strands of DNA to relieve tension at supercoils e. places RNA primers at their proper location on the template strands f. acts as starting points for DNA polymerase g. adds DNA nucleotides to form new DNA strands h. forms phosphodiester bonds to join Okazaki fragments Esc 1. single-strand binding protein 2. helicase 3. DNA ligase 4. RNA primer 5. gyrase 6. DNA polymerase 53°F Cloudy 3 Q Search $ F5 FRarrow_forwardWhich of the following is NOT true of DNA replication? a. Helicase uses ATP as an energy source. b. Primase uses ATP to build primers. c. Single-stranded binding proteins are enzymes that hold the strands open. d. Telomerase adds nucleotides to the original DNA template. e. Ligase uses a condensation reaction to make a single phosphodiester bond at each site of ligation..arrow_forwardWhich of the following statements is TRUE concerning the synthesis of the leading and lagging strands of DNA in prokaryotic cells? a. O b. The leading strand is synthesized by one polymerase III continuously, and the lagging strand is synthesized by several molecules of DNA polymerase III. d. The leading and lagging strands are synthesized at the same time by the one DNA polymerase I. O c. The leading and lagging strands are synthesized at the same time by the one DNA polymerase III. The leading strand is synthesized by one polymerase III, and the lagging strand is synthesized by DNA polymerase I.arrow_forward
- The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.arrow_forwardWould it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. B. No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. C. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first.arrow_forwardMatch the terms associated with the polymerase chain reaction with their correct descriptions. Refers to the fact that DNA molecules get longer the more of them there are in the reaction. A. В. Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other. Denaturation C. Incubate the reaction at the optimal temperature for the primers to base-pair with each other. Annealing. D. Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence. Extension. Add a chaotropic agent that destabilizes hydrogen bonding. E. Amplification. F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C). G. Happens after repeated cycles of the temperature change regimen. Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen. Н.arrow_forward
- Which of the following is NOT true of DNA polymerase? a. DNA polymerase builds the new strand from 5' to 3'. b. DNA polymerase can start new DNA strands independently. c. DNA polymerase uses a "guess and check" method to add nucleotides. d. DNA polymerase uses dATP as a nucleotide. e. DNA polymerase reads the template from 3' to 5'.arrow_forwardWhich of the following statements is not true? Explain why. A. A DNA strand can serve as a template strand on many occasions. B. Following semiconservative DNA replication, one strand is a newly made daughter strand and the other strand is a parental strand. C. A DNA double helix may contain two strands of DNA that were made at the same time. D. A DNA double helix obeys the AT/GC rule. E. A DNA double helix could contain one strand that is 10 generations older than its complementary strand.arrow_forwardFor the statements below, indicate whether the statement applies to the leading strand, or to the lagging strand, or to both. a. synthesized in the 5'→3' direction b. synthesized continuously c. require(s) DNA ligase to join fragments d. described as a daughter strand e. synthesized in the same direction as the movement of the replication fork f. DNA polymerase is the enzyme involved in forming this polynucleotide.arrow_forward
- Match the enzymes provided from (1-4) in the list of choices with their matching function (A-D) during DNA replication. A. Disrupts hydrogen bonds between DNA bases B. Can only add nucleotides to an existing 3 OH end C. Can't add nucleotides to a chain, but can make covalent bonds D. Actually a specialized form of RNA polymerase select 1. DNA polymerase select 2. Primase select 3. Ligase select v 4. Helicasearrow_forwardThe use of dideoxynucleotide triphosphates in the Sanger method facilitates sequencing of polynucleotides. Why? A. Fragments are generated with the terminal ends corresponding to the complement of the DNA being sequenced. B. Incorporation of the specific dideoxynucleotides terminates polymerization of the newly synthesized DNA. C. Dideoxynucleotides are randomly incorporated to the growing polypeptide chain D. Both A and B E. All of thesearrow_forwardWhich of the following is/are not required for DNAreplication to occur?a. DNA polymerase d. primersb. nucleotides e. helicasec. template DNA f. all are requiredarrow_forward
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