Concept explainers
a.
To predict:
The possible outcome of Griffith’s experiment using the heat-killed pathogenic and heat killed nonpathogenic bacteria.
Introduction:
The transformation of the genetic material in bacteria was experimentally found out by the person named F. G. He was trying to make the vaccine that could cure pneumonia, which is caused by the bacteria Streptococcus pneumonia. There exist two different strains of this bacterium. One of the forms of the bacteria is known as the S (smooth) form. This strain of the bacteria is virulent and forms smooth colonies in the culture. The other form is the mutant form or the R (rough) form. This strain was non-virulent and forms a rough colony in the culture.
b.
To determine:
The possible outcome of Griffith’s experiment using the heat-killed pathogenic and live nonpathogenic in the presence of an enzyme that digests proteins (protease).
Introduction:
The transformation of the genetic material in bacteria was experimentally found out by the person named F. G. He was trying to make the vaccine that could cure pneumonia, which is caused by the bacteria Streptococcus pneumonia. There exist two different strains of this bacterium. One of the forms of the bacteria is known as the S (smooth) form. This strain of the bacteria is virulent and forms smooth colonies in the culture. The other form is the mutant form or the R (rough) form. This strain was non-virulent and forms a rough colony in the culture.
c.
To determine:
The possible outcome of Griffith’s experiment using the heat-killed pathogenic and live nonpathogenic in the presence of an enzyme that digests DNA (endonuclease).
Introduction:
The transformation of the genetic material in bacteria was experimentally found out by the person named F. G. He was trying to make the vaccine that could cure pneumonia, which is caused by the bacteria Streptococcus pneumonia. There exist two different strains of this bacterium. One of the forms of the bacteria is known as the S (smooth) form. This strain of the bacteria is virulent and forms smooth colonies in the culture. The other form is the mutant form or the R (rough) form. This strain was non-virulent and forms a rough colony in the culture.
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- In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain pneumonia solutions were added to non-virulent R Strain pneumonia cell cultures and allowed to grow in liquid media (i.e., broth). In tubes where Protease was added to the supernatant prior to cell culture, what was the observed effect when plating and growing the S. pneumonia cells to solid media? Selected answer will be automatically saved. For keyboard navigation, press up/down arrow keys to select an answer. a b C d e All RNA was degraded and Transformation of the R Strain to S Strain occurred. All Protein was degraded and Transformation of the R Strain to S Strain occurred. All DNA was degraded and Transformation of the R Strain to S Strain occurred. All RNA was degraded and no Transformation occurred indicating RNA is the molecule of Transformation inheritance None of the above are truearrow_forwardCite the qualitative tests to characterize the products of RNA hydrolysis. Indicate the reagents involved in the process and indicate the positive result: A. Bial's test B.Murexide Testarrow_forwardDescribe and explain the difference in results if the serial dilution step of the T4 bacteriophage suspension inour experiment was skipped (i.e., all of Step #3 of the procedures was skipped)? _________________________Current EventsThe Johnson and Johnson COVID-19 vaccine consists of inactivated adenovirus that has been altered to containgenetic information encoding SARS-CoV-2 surface spike proteins. The virus targets and delivers this geneticinformation to the cells of our respiratory tract, which produce benign SARS-CoV-2 surface spike proteins butare effective for training our immune system to defend us against SARS-CoV-2.Based on this information and your knowledge of viruses in general, could T4 bacteriophage be used in place ofarrow_forward
- In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain pneumonia solutions were added to non-virulent R Strain pneumonia cell cultures and allowed to grow in liquid media (i.e., broth). In tubes where Protease was added to the supernatant prior to cell culture, what was the observed effect when plating and growing the S. pneumonia cells to solid media?arrow_forwardWhat is the Kirby-Bauer Test? Describe it and explain how you would interpret the results.arrow_forwardMatch the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fractionarrow_forward
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