Concept explainers
A process by which a polypeptide chain folds to form an active protein is known as protein folding. The structure of a protein is very critical for its function. A process by which a protein molecules move between cellular compartments is known as protein translocation. The translocation process advances the mRNA-tRNA moiety on the ribosome, in order to permit the next codon to enter into the decoding center. There are two translocation systems such as Sec system and Tat system and two secretion systems, such as Type I and Type IV are present in both Gram-positive bacteria and Gram-negative bacteria.
The translocation or secretion system that is most widespread of all the translocation system is the Sec system. The Sec system is occasionally termed as the general secretion pathway. This is because, it is highly conserved and have been identified in all three domains of life, such as eukaryotes, bacteria, and archaea. The Sec system translocates unfolded proteins across the plasma membrane or incorporates the unfolded proteins in the plasma membrane itself.
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Prescott's Microbiology
- Protein analysis by gel electrophoresis a). Using the gel image provided to calculate the electrophoretic relative mobility as a ratio of the distance of protein migration to the distance of the tracking dye migration (See Appendix B). If your dye front is not visible, measure the mobility relative to the bottom edge of the resolving gel. Include a labeled print-out of your gel image with your report. b). Plot log MW of the protein markers and commercial myoglobin vs. mobility. Determine the molecular weight of myoglobin obtained after the final purification step (Sample E) from the equation of the line. Submit a copy of your graph along with the gel image. c). Compare your sample E and commercial myoglobin with the ladder. Summary your results. order of sample (left to right) is : A B C D blank E blank Commercial Myoglobin Molecular Ladderarrow_forwardGive Detailed Solution (no need Handwritten)arrow_forwardtopic: sds-page gel What is the purpose of adding APS and TEMEDarrow_forward
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