What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and then grown in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium. Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

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Chapter 13, Problem 17TYU

Summary Introduction

To explain: What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and then grown in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium.

Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

Summary Introduction

To explain: What happens when Neurospora spores are exposed to X-rays and a culture is established from the irradiated spores and if the fungus does not grow in (1) a minimal medium plus vitamins, (2) a minimal medium plus amino acids, and (3) a minimal medium.

Introduction: Genes are sets of nucleotides that code for a particular protein. The genes have to be expressed for the synthesis of a protein. This gene expression is regulated depending on the requirement of the cell. For example, cell division proteins are synthesized prior to the cell division. Prokaryotic and eukaryotic genes have differential gene expression in order to suit the specific needs of the cell.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:

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