Concept explainers
(a)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(b)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(c)
Interpretation:
Theabsorbance of the solution for the percent transmittance of half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(d)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(e)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(f)
Interpretation:
Theabsorbance of the solution for the percent transmittance of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
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Chapter 13 Solutions
Principles of Instrumental Analysis
- Given the following sample concentrations and corresponding absorbances, construct a standard curve of absorbance vs. concentration and determine the molar absorptivity constant. Sample Concentration 15 μΜ 25 μΜ 40 μΜ Absorbance 0.19 0.32 0.49 55 μΜ 0.70arrow_forwardBriefly explain why it is NOT accurate to calculate the concentration of a sample whose absorbance is 0.185 using the equation y=0.0022x+0.0005 r^2=0.999arrow_forwardA student prepared several solutions of an unknown molecule, each at a different concentration. The absorbance of each solution was measured at 630 nm with a path length of 0.760 cm. The student then plotted the absorbance of each sample vs. its concentration (in uM), and found a best-fit line of y = 0.00626x + 0.00732. Calculate the molar absorptivity of the unknown molecule (in M-1 cm-1).arrow_forward
- 2arrow_forward4 of 15 Abs 2.0 1.5 1.0 0.5 0.25 0. 0.2 0.4 0.6 Concentration (M) With reference to the UV-Vis calibration curve reported above, calculate the concentration (Molar) of a sample whose Absorbance is 1.0. Report your answer with two significant figures.arrow_forwardA solution prepared by dissolving 25.8 mg of benzene (78.11 g/mol) in hexane (86.16 g/mol) and diluting to 250 mL had an absorption peak at 256 nm and an absorbance of 0.266 in a 1.000-cm celI. What is the percent transmittance? O 1.84% O 184% O 54.2% O 0.542%arrow_forward
- A 1.3 * 10-5M solution of an unknown substance has an absorbance of 0.345 at its λmax. Calculate the molar absorbtivity, ℇ, of the substance at this wavelength, using a sample cell with 1.00cm path length. Then calculate the absorbance of an aqueous solution of this substance at its λmax if its concentration is 2.5 * 10-5M.arrow_forward(d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?arrow_forwardA standard curve of absorbance vs. concentration has a linear trendline of y = 0.56x + 0.987. Use this trendline to calculate the concentration of an unknown sample that has an absorbance of 1.245.arrow_forward
- In a 1.00 cm cell, a colored solution transmits 40.0% of the incident light that passed through it. ε = 4.52 x 10³ Lmol-¹cm-¹. Determine absorbance of the solution 0.398 0.220 60.00 0.400arrow_forwardWhy is it most accurate to measure absorbances in the range A = 0.3 to 2?arrow_forwardA 2.54 x 10-M sample of compound X in a solvent has an absorbance of 0.370 at 528 nm in a 1.000 cm cuvette. The solvent alone has an absorbance of 0.058. What is the molar absorptivity of compound X? € = M-1.cm- A different of compound X in the same solvent has an absorbance of 0.475 at 528 nm when measured with the same cuvette. What is the concentration of this second sample? mol X [X] = L * TOOLS |x10arrow_forward
Macroscale and Microscale Organic ExperimentsChemistryISBN:9781305577190Author:Kenneth L. Williamson, Katherine M. MastersPublisher:Brooks Cole
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