As several absorbance values have been given under 375 nm, the most accurate way is to construct a graph between absorbance & the concentration of CrO 42- solution. After using the value of slope & considering the chemical equation of Beer-Lambert law, absorptivity and the molar absorptivity of CrO 42- should be calculated. Concept introduction: The absorbance of light can be determined by the Beer-Lambert law. The chemical equation relevant to the law is, A = c l ε

Question

(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

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Question

Chapter 13, Problem 13.24QAP

Interpretation Introduction

Interpretation:

As several absorbance values have been given under 375 nm, the most accurate way is to construct a graph between absorbance & the concentration of CrO_{42- solution. After using the value of slope & considering the chemical equation of Beer-Lambert law, absorptivity and the molar absorptivity of CrO_{42- should be calculated.}}

Concept introduction:

The absorbance of light can be determined by the Beer-Lambert law. The chemical equation relevant to the law is,

A=clε

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