Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Question

Want to see the full answer?

Answer 1

Question

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

What is cloning? How is it done?

What are the origins and functions of gene clusters?

What are gene chips ?

Answer 2

Question

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Answer 6

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Answer 7

Chapter 12.3, Problem 3MQ

Summary Introduction

Protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins may be toxic to the host and protease enzymes may degrade other protein molecules. In addition, overproduction of proteins may induce protein aggression and that lead to insoluble inclusions. Through, the purification of inclusion are relatively easy, solubilizing the inclusion containing protein is difficult. Also, they can be denatured partially. The purification of protein is easy if the protein is formed as a fusion protein with carrier protein, which is encoded by a vector.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQ Ch. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQ Ch. 12.2 - Prob. 3MQ Ch. 12.2 - Prob. 1CR Ch. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...

Solution Summary: The author explains that protein synthesis in a host organism for molecular cloning purposes may cause problems. Some proteins are toxic to the host and protease enzymes may degrade other protein molecules.

Concept explainers

Videos

Want to see the full answer?

Chapter 12 Solutions