Brock Biology of Microorganisms (15th Edition)

15th Edition

ISBN: 9780134261928

Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl

Publisher: PEARSON

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establ…

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Chapter 12.2, Problem 1MQ

What is the purpose of molecular cloning?

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Chapter 12.1, Problem 1CR

Chapter 12.2, Problem 2MQ

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Chapter 12 Solutions

Brock Biology of Microorganisms (15th Edition)

Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQ Ch. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQ Ch. 12.2 - Prob. 3MQ Ch. 12.2 - Prob. 1CR Ch. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...

Ch. 12.3 - Prob. 3MQ Ch. 12.3 - Prob. 1CR Ch. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQ Ch. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQ Ch. 12.6 - Prob. 2MQ Ch. 12.6 - Prob. 3MQ Ch. 12.6 - Prob. 1CR Ch. 12.7 - Prob. 1MQ Ch. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQ Ch. 12.8 - Prob. 3MQ Ch. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQ Ch. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQ Ch. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CR Ch. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQ Ch. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CR Ch. 12.12 - Prob. 1MQ Ch. 12.12 - Prob. 2MQ Ch. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQ Ch. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQ Ch. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQ Ch. 12 - Prob. 3AQ Ch. 12 - Describe how you could recode Escherichia coli to...

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What is the purpose of molecular cloning?

Solution Summary: The author explains that molecular cloning is used to produce recombinant DNA, which contains DNA from various sources, and then inserted into a host for replication.

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