
Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Question
Chapter 12, Problem 2AQ
Summary Introduction
To discuss:
Several genetic systems use the β-galactosidase as a reporter. The lacZ gene encodes β-galactosidase. The major advantages and disadvantages of using the luciferase or green fluorescent protein as a reporter instead of the β-galactosidase.
Concept introduction:
The lacZ gene from E. coli encodes β-galactosidase enzyme, which is required for catabolism of lactose. The cells are plated on X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside) containing media. Cells expressing β-galactosidase enzyme can be analyzed by their color of the colonies. X-gal, the chemical substance is cleaved by β-galactosidase enzyme and produces blue color colonies.
Expert Solution & Answer

Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?
What are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?
Why cells go through various types of cell division and how eukaryotic cells control cell growth through the cell cycle control system?
Chapter 12 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
Knowledge Booster
Similar questions
- In one paragraph show how atoms and they're structure are related to the structure of dna and proteins. Talk about what atoms are. what they're made of, why chemical bonding is important to DNA?arrow_forwardWhat are the structure and properties of atoms and chemical bonds (especially how they relate to DNA and proteins).arrow_forwardThe Sentinel Cell: Nature’s Answer to Cancer?arrow_forward
- Molecular Biology Question You are working to characterize a novel protein in mice. Analysis shows that high levels of the primary transcript that codes for this protein are found in tissue from the brain, muscle, liver, and pancreas. However, an antibody that recognizes the C-terminal portion of the protein indicates that the protein is present in brain, muscle, and liver, but not in the pancreas. What is the most likely explanation for this result?arrow_forwardMolecular Biology Explain/discuss how “slow stop” and “quick/fast stop” mutants wereused to identify different protein involved in DNA replication in E. coli.arrow_forwardMolecular Biology Question A gene that codes for a protein was removed from a eukaryotic cell and inserted into a prokaryotic cell. Although the gene was successfully transcribed and translated, it produced a different protein than it produced in the eukaryotic cell. What is the most likely explanation?arrow_forward
- Molecular Biology LIST three characteristics of origins of replicationarrow_forwardMolecular Biology Question Please help. Thank you For E coli DNA polymerase III, give the structure and function of the b-clamp sub-complex. Describe how the structure of this sub-complex is important for it’s function.arrow_forwardMolecular Biology LIST three characteristics of DNA Polymerasesarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning

Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning

Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning

Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning

Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
