Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 12.12, Problem 2MQ
Summary Introduction
Genome editing is the insertion, deletion, or modification of DNA at a specific site in the genome. In genome editing, the CRISPR/Cas9 (CRISPR associated protein 9) system recognizes and cleaves a particular DNA sequence. Optimization of this system helps in the
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
For short answer
questions, write your
answers on the line
provided. To the right is
the mRNA codon table
to use as needed
throughout the exam.
First letter
U
บบบ
U CA
UUCPhe
UUA
UCU
Phe UCC
UUG Leu
CUU
UAU.
G
U
UAC TV UGCys
UAA Stop UGA Stop A
UAG Stop UGG Trp
Ser
UCA
UCG
CCU]
0
CUC
CUA
CCC
CAC
CAU His
CGU
CGC
Leu
Pro
CCA
CAA
Gin
CGA Arg
CUG
CCG
CAG
CGG
AUU
ACU
AAU T
AUC lle
A
1
ACC
Thr
AUA
ACA
AUG
Mot ACG
AGG Arg
GUU
GCU
GUC
GCC
G
Val
Ala
GAC Asp
GGU
GGC
GUA
GUG
GCA
GCG
GAA
GGA
Gly
Glu
GAGJ
GGG
AACASH AGU Ser
AAA1
AAG Lys
GAU
AGA
CAL CALUCAO CAO
G
Third letter
1. (+7) Use the table below to answer the questions; use the codon table above to assist you.
The promoter sequence of DNA is on the LEFT. You do not need to fill in the entire table.
Assume we are in the middle of a gene sequence (no need to find a start codon).
DNA
1
DNA
2
mRNA
tRNA
Polypeptide
C
Val
G
C.
T
A
C
a. On which strand of DNA is the template strand (DNA 1 or 2)?_
b. On which side of the mRNA is the 5' end (left or…
3. (6 pts) Fill in the boxes according to the directions on the right.
Structure
R-C
R-COOH
OH
R-OH
i
R-CO-R' R
R-PO4
R-CH3
C.
0
R'
R-O-P-OH
1
OH
H
R-C-H
R-N'
I-
H
H
R-NH₂
\H
Name
Properties
4. (6 pts) Use the molecule below to answer these questions and identify the side chains and
ends. Please use tidy boxes to indicate parts and write the letter labels within that box.
a. How many monomer subunits are shown?
b. Box a Polar but non-ionizable side chain and label P
c. Box a Basic Polar side chain and label BP
d. Box the carboxyl group at the end of the polypeptide and label with letter C (C-terminus)
H H OHHO
H
H
0
HHO
H-N-CC-N-C-C
N-C-C-N-GC-OH
I
H-C-H
CH2
CH2
CH2
H3C-C+H
CH2
CH2
OH
CH
CH₂
C=O
OH
CH2
NH2
Chapter 12 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- please draw in what the steps are given. Thank you!arrow_forwardplease draw in and fill out the empty slots from image below. thank you!arrow_forwardThere is a species of eagle, which lives in a tropical forest in Brazil. The alula pattern of its wings is determined by a single autosomal gene with four alleles that exhibit an unknown hierarchy of dominance. Genetic testing shows that individuals 1-1, 11-4, 11-7, III-1, and III-4 are each homozygous. How many possible genotypes among checkered eagles in the population?arrow_forward
- students in a science class investiged the conditions under which corn seeds would germinate most successfully. BAsed on the results which of these factors appears most important for successful corn seed germination.arrow_forwardI want to write the given physician orders in the kardex formarrow_forwardAmino Acid Coclow TABle 3' Gly Phe Leu (G) (F) (L) 3- Val (V) Arg (R) Ser (S) Ala (A) Lys (K) CAG G Glu Asp (E) (D) Ser (S) CCCAGUCAGUCAGUCAG 0204 C U A G C Asn (N) G 4 A AGU C GU (5) AC C UGA A G5 C CUGACUGACUGACUGAC Thr (T) Met (M) lle £€ (1) U 4 G Tyr Σε (Y) U Cys (C) C A G Trp (W) 3' U C A Leu בוט His Pro (P) ££ (H) Gin (Q) Arg 흐름 (R) (L) Start Stop 8. Transcription and Translation Practice: (Video 10-1 and 10-2) A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense DNA strand and label the 5' and 3' ends. B. Use the antisense strand that you create in part A as a template to create the mRNA transcript of the gene and label the 5' and 3' ends. C. Translate the mRNA you produced in part B into the polypeptide sequence making sure to follow all the rules of translation. 5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand) 4arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
Case Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License