EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 12, Problem 5TMW
Summary Introduction
To Tell:
How does the scientist know that water molds are more closely related to brown algae than to the true molds.
Introduction:
Water molds are the protists which have filamentous structures resembling
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 12 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 12 - Why is it incorrect to call mitosis cell division?Ch. 12 - Why did early taxonomists categorize such...Ch. 12 - Prob. 1EDCSCh. 12 - Prob. 3TMWCh. 12 - Why arent there large numbers of pathogenic algae?Ch. 12 - Prob. 5TMWCh. 12 - Prob. 6TMWCh. 12 - Haploid nuclei _________. a. contain one set of...Ch. 12 - Prob. 2MCCh. 12 - Which of the following statements accurately...
Ch. 12 - Prob. 4MCCh. 12 - Prob. 5MCCh. 12 - Prob. 6MCCh. 12 - A phycologist studies which of the following? a....Ch. 12 - The stemlike portion of a seaweed is called its...Ch. 12 - Carrageenan is found in the cell walls of which...Ch. 12 - Prob. 10MCCh. 12 - Which of the following features characterizes...Ch. 12 - Amoebas include microbes with ________. a....Ch. 12 - The motile feeding stage of a protozoan is called...Ch. 12 - Prob. 14MCCh. 12 - Prob. 15MCCh. 12 - 1. ___ Mitosis 2. ___ Meiosis 3. __ Homologous...Ch. 12 - Prob. 2MCh. 12 - Match each of the following descriptions with the...Ch. 12 - Prob. 1VICh. 12 - Prob. 2VICh. 12 - Prob. 1SACh. 12 - Prob. 2SACh. 12 - Prob. 3SACh. 12 - What are the taxonomic challenges in classifying...Ch. 12 - List several economic benefits of algae.Ch. 12 - Why are relatively large animals such as parasitic...Ch. 12 - Prob. 7SACh. 12 - What is the role of rRNA sequencing in the...Ch. 12 - Prob. 9SACh. 12 - The study of protozoa is called ________.Ch. 12 - Prob. 2FIBCh. 12 - Prob. 3FIBCh. 12 - Prob. 4FIBCh. 12 - Prob. 5FIBCh. 12 - How are cysts of protozoa similar to bacterial...Ch. 12 - Prob. 2CTCh. 12 - Why doesnt penicillin act against any of the...Ch. 12 - Prob. 4CTCh. 12 - Prob. 5CTCh. 12 - Prob. 6CTCh. 12 - Which metabolic pathways are present in protozoa...Ch. 12 - Prob. 8CTCh. 12 - Prob. 9CTCh. 12 - Prob. 10CTCh. 12 - Prob. 11CTCh. 12 - Prob. 12CTCh. 12 - Using the following terms, fill in the following...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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