The virus does not contain ribosomes. The mechanism through which it manages to ensure the synthesis of proteins is to be discussed. Concept introduction: Viruses are the infectious agents that infect the living cell of the organism. They infect the cells, utilize its machinery, and replicate inside the living cell of an organism. They have the capability to infect any type of organism, from plants to animals, and microorganisms like bacteria and archaebacteria. They are nonliving entities outside the living body and become active inside the living cell. Thus, they are the link between the living entities and the nonliving entities.

Question

The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

Answer 1

Question

Chapter 12, Problem 43RE

Interpretation Introduction

Interpretation:

The virus does not contain ribosomes. The mechanism through which it manages to ensure the synthesis of proteins is to be discussed.

Concept introduction:

Viruses are the infectious agents that infect the living cell of the organism. They infect the cells, utilize its machinery, and replicate inside the living cell of an organism.

They have the capability to infect any type of organism, from plants to animals, and microorganisms like bacteria and archaebacteria. They are nonliving entities outside the living body and become active inside the living cell. Thus, they are the link between the living entities and the nonliving entities.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!