Owlv2,1 Term Printed Access Card For Campbell/farrell/mcdougal's Biochemistry, 9th
9th Edition
ISBN: 9781305962972
Author: Campbell, Mary K.; Farrell, Shawn O.; Mcdougal, Owen M.
Publisher: Cengage Learning
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Chapter 12, Problem 41RE
Interpretation Introduction
Interpretation:
The detailed information about ribosomal structure by electron microscopy can be explained.
Concept introduction:
Electron microscopy is a type of technique used to obtain the high-resolution images of biological and nonbiological specimens. It is used to investigate the structure of tissues, cells, and macromolecular complexes.
X-ray crystallography is a type of technique used for structure determination of proteins and biological macromolecules. This technique is currently the most favored technique.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 12 Solutions
Owlv2,1 Term Printed Access Card For Campbell/farrell/mcdougal's Biochemistry, 9th
Ch. 12 - RECALL Prepare a flow chart showing the stages of...Ch. 12 - Prob. 2RECh. 12 - RECALL Define degenerate code.Ch. 12 - RECALL How can the binding assay technique be used...Ch. 12 - RECALL Which nucleotides break the rules of...Ch. 12 - Prob. 6RECh. 12 - Prob. 7RECh. 12 - REFLECT AND APPLY It is possible for the codons...Ch. 12 - Prob. 9RECh. 12 - Prob. 10RE
Ch. 12 - REFLECT AND APPLY How would protein synthesis be...Ch. 12 - REFLECT AND APPLY Comment on the evolutionary...Ch. 12 - Prob. 13RECh. 12 - Prob. 14RECh. 12 - RECALL What is the role of ATP in amino acid...Ch. 12 - Prob. 16RECh. 12 - Prob. 17RECh. 12 - Prob. 18RECh. 12 - REFLECT AND APPLY A friend tells you that she is...Ch. 12 - Prob. 20RECh. 12 - REFLECT AND APPLY Is amino acid activation...Ch. 12 - Prob. 22RECh. 12 - Prob. 23RECh. 12 - Prob. 24RECh. 12 - RECALL What are the A site and the P site? How are...Ch. 12 - Prob. 26RECh. 12 - RECALL Describe the role of the stop signals in...Ch. 12 - Prob. 28RECh. 12 - RECALL What is the ShineDalgarno sequence? What...Ch. 12 - REFLECT AND APPLY You are studying with a friend...Ch. 12 - REFLECT AND APPLY E. coli has two tRNAs for...Ch. 12 - REFLECT AND APPLY In prokaryotic protein...Ch. 12 - REFLECT AND APPLY Describe the recognition process...Ch. 12 - REFLECT AND APPLY The fidelity of protein...Ch. 12 - REFLECT AND APPLY (a) How many activation cycles...Ch. 12 - REFLECT AND APPLY What is the energy cost per...Ch. 12 - Prob. 37RECh. 12 - Prob. 38RECh. 12 - Prob. 39RECh. 12 - Prob. 40RECh. 12 - Prob. 41RECh. 12 - Prob. 42RECh. 12 - Prob. 43RECh. 12 - Prob. 44RECh. 12 - Prob. 45RECh. 12 - Prob. 46RECh. 12 - Prob. 47RECh. 12 - RECALL What are two major similarities between...Ch. 12 - REFLECT AND APPLY Why do amino acids other than...Ch. 12 - REFLECT AND APPLY Would puromycin be useful for...Ch. 12 - Prob. 51RECh. 12 - Prob. 52RECh. 12 - Prob. 53RECh. 12 - Prob. 54RECh. 12 - Prob. 55RECh. 12 - Prob. 56RECh. 12 - REFLECT AND APPLY The amino acid hydroxyproline is...Ch. 12 - Prob. 58RECh. 12 - Prob. 59RECh. 12 - Prob. 60RECh. 12 - Prob. 61RECh. 12 - Prob. 62RECh. 12 - Prob. 63RECh. 12 - Prob. 64RECh. 12 - Prob. 65RECh. 12 - Prob. 66RECh. 12 - Prob. 67RECh. 12 - Prob. 68RECh. 12 - Prob. 69RECh. 12 - Prob. 70RECh. 12 - Prob. 71RECh. 12 - Prob. 72RECh. 12 - Prob. 73RE
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