Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 11.3, Problem 19AYP
Summary Introduction
To define:
The terms “incidence” and “prevalence” and also differentiate them.
Concept introduction:
In epidemiology, patterns of disease can be studied along with the effect of the disease on health of people. There are three types of diseases based on the area that are, endemic, epidemic and pandemic. Epidemiologists identify the “prevalence” of a disease.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 11 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 11.1 - Prob. 1AYPCh. 11.1 - Enumerate the sites where normal biota is found in...Ch. 11.1 - Discuss how the Human Microbiome Project is...Ch. 11.1 - Prob. 2NPCh. 11.2 - Differentiate between a microbes pathogenicity and...Ch. 11.2 - Prob. 5AYPCh. 11.2 - Explain the significance of polymicrobial...Ch. 11.2 - Prob. 7AYPCh. 11.2 - Prob. 8AYPCh. 11.2 - Prob. 9AYP
Ch. 11.2 - Prob. 10AYPCh. 11.2 - Prob. 11AYPCh. 11.2 - Draw a diagram of the stages of disease in a humanCh. 11.2 - Prob. 13AYPCh. 11.2 - Prob. 14AYPCh. 11.2 - Define healthcare-associated infection, and list...Ch. 11.2 - Prob. 16AYPCh. 11.2 - Prob. 1MMCh. 11.2 - Prob. 3NPCh. 11.2 - Prob. 2MMCh. 11.2 - Prob. 4NPCh. 11.2 - Prob. 5NPCh. 11.2 - Q. Can you think of what type of incident might...Ch. 11.2 - Prob. 6NPCh. 11.3 - Summarize the goals of epidemiology, and...Ch. 11.3 - Prob. 18AYPCh. 11.3 - Prob. 19AYPCh. 11.3 - Discuss the three major types of epidemics, and...Ch. 11 - Prob. 1NPCh. 11 - Prob. 1QCh. 11 - Prob. 2QCh. 11 - Prob. 3QCh. 11 - Prob. 4QCh. 11 - Prob. 5QCh. 11 - Discuss the role of endospores in ensuring the...Ch. 11 - Prob. 7QCh. 11 - Correlate the stages in the course of an...Ch. 11 - Prob. 9QCh. 11 - Prob. 10QCh. 11 - Prob. 11QCh. 11 - Prob. 12QCh. 11 - Prob. 13QCh. 11 - Prob. 14QCh. 11 - Prob. 15QCh. 11 - Prob. 16QCh. 11 - Prob. 17QCh. 11 - Prob. 18QCh. 11 - The number of cases, including new cases as well...Ch. 11 - Prob. 20QCh. 11 - One important way to critically analyze a study...Ch. 11 - Prob. 1VC
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