The physical control methods for disinfection. Introduction: Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

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Answer 1

Question

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

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a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________

1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

Answer 2

Question

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Expert Solution & Answer

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Answer 3

Question

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Expert Solution & Answer

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Answer 4

Question

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Expert Solution & Answer

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Answer 5

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Answer 6

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Answer 7

Chapter 11, Problem 2CC

Summary Introduction

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Answer 8

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Answer 9

Summary Introduction

To determine:

The types of materials required for disinfection, not for sterilization.

Introduction:

Disinfection is a process of removal cleaning of microorganisms from the surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agents.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 11.1 - Distinguish among the terms sterilization,...Ch. 11.1 - Prob. 2AYP Ch. 11.1 - Prob. 3AYP Ch. 11.1 - Name four categories of cellular targets for...Ch. 11.2 - Prob. 5AYP Ch. 11.2 - Prob. 6AYP Ch. 11.2 - Define thermal death time and thermal death point,...Ch. 11.2 - Prob. 8AYP Ch. 11.2 - Prob. 9AYP Ch. 11.2 - Identify advantages and disadvantages of cold...

The physical control methods for disinfection. Introduction: Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

To determine: The physical control methods for disinfection. Introduction: Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.

Want to see the full answer?

Chapter 11 Solutions

To determine:

The physical control methods for disinfection.

Introduction:

Disinfection is a process of removal of microorganisms from a surface. It is done by using disinfectants. The disinfectants destroy, inactivate or kill microorganisms. It is a type of chemical agent.