EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
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Chapter 11, Problem 18P
Summary Introduction
To determine:
The ways by which a prosecutor use DNA from the seed pods to build a case against the defendant.
Introduction:
DNA fingerprinting is defined as the method in which variable elements present in the sequence of DNA are isolated and identified. The DNA is obtained from a sample of cells such as skin, blood, or hair cells. They are a rich source of DNA.
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The Evidence shows which suspect was at the crime scene? ( image of murder investigation)
A) suspect A
B) suspect B
C) suspect C
D) none were at the crime scene
Which of the following choices most likely lists the sizes of the four DNA fragments in lane 6 ( second image) of this agarose gel stained with ethidium bromide? ( Note: the well that the DNA was loaded into is at the top of the gel image).
A) 100 bp, 300 bp, 450 bp, 600 bp.
B) 300 bp, 400 bp, 100 bp, 600 bp
C) 600 bp, 450 bp, 300 bp, 100 bp
D) 400 bp, 300 bp, 600 bp, 100 bp
E) DNA fragment size can't be estimated using an agarose gel.
Brenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break?
A. Obtaining and handling DNA sample without wearing googles or gloves
B. Improper use of human DNA samples
C. Violating Patient Confidentiality
D. Disposing of bio hazardous material in a regular trash
Below is an image of the results of a gel electrophoresis experiment.
Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the
DNA size marker (DSM). From this image and by only using Lanes 1-
4 which lane matches each description below?
a) This lane contains the largest DNA fragment:
b) This lane contains the smallest DNA fragment:
c) This lane contains a 2.5 kbp DNA fragment:
42
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||| | ||
12 kb
9.5 kb
6.5 kb
4 kb
2 kb
***
Chapter 11 Solutions
EBK GENETICS: FROM GENES TO GENOMES
Ch. 11 - Choose the phrase from the right column that best...Ch. 11 - Would you characterize the pattern of inheritance...Ch. 11 - Would you be more likely to find single nucleotide...Ch. 11 - A recent estimate of the rate of base...Ch. 11 - If you examine Fig. 11.5 closely, you will note...Ch. 11 - Approximately 50 million SNPs have thus far been...Ch. 11 - Mutations at simple sequence repeat SSR loci occur...Ch. 11 - Humans and gorillas last shared a common ancestor...Ch. 11 - In 2015, an international team of scientists...Ch. 11 - Using PCR, you want to amplify an approximately 1...
Ch. 11 - Prob. 11PCh. 11 - The previous problem raises several interesting...Ch. 11 - You want to make a recombinant DNA in which a PCR...Ch. 11 - You sequence a PCR product amplified from a...Ch. 11 - Prob. 15PCh. 11 - The trinucleotide repeat region of the Huntington...Ch. 11 - Sperm samples were taken from two men just...Ch. 11 - Prob. 18PCh. 11 - a. It is possible to perform DNA fingerprinting...Ch. 11 - On July 17, 1918, Tsar Nicholas II; his wife the...Ch. 11 - The figure that follows shows DNA fingerprint...Ch. 11 - Microarrays were used to determine the genotypes...Ch. 11 - A partial sequence of the wild-type HbA allele is...Ch. 11 - a. In Fig. 11.17b, PCR is performed to amplify...Ch. 11 - The following figure shows a partial microarray...Ch. 11 - Scientists were surprised to discover recently...Ch. 11 - The microarray shown in Problem 25 analyzes...Ch. 11 - The figure that follows shows the pedigree of a...Ch. 11 - One of the difficulties faced by human geneticists...Ch. 11 - Now consider a mating between consanguineous...Ch. 11 - The pedigree shown in Fig. 11.22 was crucial to...Ch. 11 - You have identified a SNP marker that in one large...Ch. 11 - The pedigrees indicated here were obtained with...Ch. 11 - Approximately 3 of the population carries a mutant...Ch. 11 - The drug ivacaftor has recently been developed to...Ch. 11 - In the high-throughput DNA sequencing protocol...Ch. 11 - A researcher sequences the whole exome of a...Ch. 11 - As explained in the text, the cause of many...Ch. 11 - Figure 11.26 portrayed the analysis of Miller...Ch. 11 - A research paper published in the summer of 2012...Ch. 11 - Table 11.2 and Fig. 11.27 together portray the...Ch. 11 - The human RefSeq of the entire first exon of a...Ch. 11 - Mutations in the HPRT1 gene in humans result in at...Ch. 11 - Prob. 44P
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- In typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is not the source of that sample,whereas a DNA match does not necessarily prove that a suspect is the source?arrow_forwardPlease answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardWhich of the following statements regarding DNA fingerprinting is false?a) DNA fingerprinting cannot be used for paternity testingb) DNA profile using STR (short terminal repeats) is unique to an individualc) PCR is used for DNA profilingd) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individualsarrow_forward
- Why is ripe banana a better sample compared to an unripe banana for DNA extraction ?arrow_forwardexplain with details not short answer i need so long answer with all details included table may be used.arrow_forwardIn a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y and Brother X, there is a sample of DNA from a crime scene that is likely to belong to the guilty party. Based on the gel photo below, which shows the results of an electrophoresis gel following PCR amplification at one locus of 5 DNA samples - one crime scene sample and 4 suspects - which suspects can be excluded from this investigation? [Keep in mind that it is not possible for a heterozygous person to leave only one allele at a crime scene. If any one allele does not match, then that suspect is eliminated.] choose all that applyarrow_forward
- In a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y and Brother X, there is a sample of DNA from a crime scene that is likely to belong to the guilty party. Based on the gel photo below, which shows the results of an electrophoresis gel following PCR amplification at one locus of 5 DNA samples - one crime scene sample and 4 suspects - which suspects can be excluded from this investigation? [Keep in mind that it is not possible for a heterozygous person to leave only one allele at a crime scene. If any one allele does not match, then that suspect is eliminated.] Choose all that apply.arrow_forwardTo pinpoint a Criminal, Forensic Department uses the technique called? A) DNA Editing B) DNA Splicing C) DNA Fingerprinting D) DNA Amplificationarrow_forwardQuestion 1 Being able to associate a QTL with a marker relies on linkage. Suppose researchers genotype a population for two marker loci that they think are on the same chromosome. Using these two markers they notice that out of 1,000 genotypes, there were 53 recombinant individuals. a) Make a conservative estimate of how far apart the two loci are. b) Presuming these are mammals, how many DNA bases does this distance represent?arrow_forward
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