Concept explainers
Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.
To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.
Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.
Explanation of Solution
The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.
If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.
Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.
Want to see more full solutions like this?
Chapter 10 Solutions
Concepts of Genetics (11th Edition)
- Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.arrow_forwardGive the Summary of the Hershey–Chase experiment ?arrow_forwardPlease answer fast We have lots of options when it comes to trying to purposefully make mutations while doing research. Please brieflyname/describe a method you could use to make: a) random mutations anywhere within the genome in living cells (in vivo) b) random mutations within our cloned gene of interest in vitro c) a very specific (non-random) mutation within our cloned gene of interest in vitro d) NOW for the answer you gave in part C, please elaborate in detail how this method of mutagenesis is performed.arrow_forward
- List and Describe the algorithm of BLOSUM substitution matrix computation.arrow_forwardGivearrow_forwardGive typed full explanation Please describe the Hershey and Chase experiment, including: a. What they were trying to learn b. How they set up their experiment c. Their results d. What they concluded, and whyarrow_forward
- What was studied or investigated in the paper? Who would be affected by this? https://opus.lib.uts.edu.au/bitstream/10453/31887/1/2012007630OK.pdfarrow_forwardWith regard to Chargaff’s experiment described in Figure shown,answer the following:A. What is the purpose of paper chromatography?B. Explain why it is necessary to remove the bases in order todetermine the base composition of DNA.C. Would Chargaff’s experiments have been convincing if theyhad been done on DNA from only one species? Discuss.arrow_forwardplease solve this with step-by-step calculations and explanations.arrow_forward
- In the experiments of Aery, MacLeod, and McCarty, what was the purpose of treating the transforming extract with enzymes?arrow_forwardDescribe one of the experiments that helped to solve the puzzle concerning the nature of the genetic material.arrow_forwardCould you explain how you would know if you successfully induced transformation? Thank you Did you successfully demonstrate transformation occurred? Why or why not? (How do I know, what do I look for?) Explain the control used in this experiment. What was it, and why was it necessary? Based on the calculation in the question above, comment on the relative difficulty of achieving successful transformation of E. coli. (Please explain the relative difficulty)arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning