Explanation The enzyme DNA polymerase consists of many subunits that help in the binding of the enzyme to the template DNA, forming a clamp and loading the clamp to the template DNA. The polymerizing part of the DNA polymerase III enzyme forms a closed ring around the DNA chain, which is called the sliding clamp. The sliding clamp helps in this process by opening the clamp as it is a closed ring and inserting the DNA into it. The beta subunits are involved in forming a clamp. This clamp proceeds along the DNA chain as polymerization occurs...

9th Edition

ISBN: 9781305961135

Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher: Cengage Learning

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 10, Problem 19RE

Interpretation Introduction

Interpretation:

Whether it is usual or not that the beta-subunits of DNA polymerase III, which forms the sliding clamp along the DNA, do not contain the active site for the polymerization reaction is to be determined.

Concept introduction:

Replication of DNA (deoxyribonucleic acid) is catalyzed by a DNA polymerase enzyme. There are many types of DNA polymerases in bacteria, of which DNA polymerase III is the main polymerase enzyme.

The DNA polymerase III has a high processivity and also has 3′ exonuclease activity. It consists of more than 10 subunits that perform various functions. These subunits include α, β γ, δ, ε, and others.

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Chapter 10 Solutions

Biochemistry

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Solution Summary: The author explains that DNA polymerase III has a high processivity and 3′ exonuclease activity. The beta subunit forms the sliding clamp, which attaches the rest of the enzyme to the template

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